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Resistance evaluation to Xylella fastidiosa of tangerine germoplasm and hibrids introduceds from Italy and Corse

The Citrus Variegated Chlorosis (CVC), caused by the Xylella fastidiosa bacteria is one of the most important diseases that affect the Brazilian citriculture, been the sweet orange the most susceptible to the bacteria. The objective of this experiment, carried out in the Bebedouro Experimental Station (EECB) in greenhouse conditions, was the evaluation of the behavior of citrus germplasm introduced by the EECB, Fundecitrus and Cenargen, in relation to the CVC. The plants were multiplicated over several rootstocks and when they achieved the right size were inoculated by grafting of diseased stem. Each material had four plants, three of them were inoculated and the other one wasn't inoculated, leaving like control. The plants were evaluated by symptoms presence, ELISA and PCR tests. The first symptoms in the contaminated materials appeared 7 months after inoculation. Eighteen materials were found positives in the PCR test, showing that this materials are susceptible to the Xylella fastidiosa. Howoever, the materials where the bacteria was reported by the ELISA, but not by the PCR test, were grouped like false positives and weren't were accepted like susceptible.

CVC; inoculation; bacteria; citrus


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