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Total phenolics and antioxidant capacity "in vitro" of tropical fruit pulps

The consumption of fruit, pulps has been highly recommended for its nutritional value, high-fiber, vitamin C and carotenoids. Recent studies have demonstrated these foods as sources of phenolic compounds with antioxidant activity, therefore, free radical scavengers, with a protective effect against the emergence anddevelopment or degenerative processes that lead to chronic diseases. Due to the increasing commercializationand consumption of fruit pulp in Brazil, especially in the city of Teresina, Piauí, this study selected a group of fruit pulp of high local consumption to evaluate the total phenolic content and antioxidant activity in vitro by the method of capture of free radicals: DPPH (1,1-radical diphenil-2-picrilhydrazil) and ABTS (2,2 'azinobis-(3-ethylbenzthiazoline-6-sulfonic acid)). The fruit were selected Acerola (Malpighia emarginata DC.) Bacuri (Platonia insignis Mart.) Caja (Spondias mombin L.), Cashew (Anacardium occidentale), Guava(Psidium guajava) and Tamarind (Tamarindus indica L.). The levels of phenolics found in the frozen pulp of fruit exhibited relevant quantities of polyphenols, especially if the pulp with 835.25 ± 32.44 and 449.63 ± 10.24 mg / 100g in hydroalcoholic and aqueous extracts, respectively followed by cashew pulp with 201.61 ± 19.15 and 165.07 ± 4.10 mg / 100g. The pulp of tamarind bacuri showed the lowest levels of total phenolics. Regarding the antioxidant activity in vitro, the best results were found for aqueous and ethanolic of pulp of acerola, cashew and guava. The antioxidant activity of these pulps (EC50 in mg / mL) rangedfrom 24.42 to 413.36 and 1.74 to 259.18 for the hydroalcoholic and aqueous extracts, respectively. Using the ABTS radical, the antioxidant activity for these same fruit pulps showed that TEAC values ranged from 3.69 ± 0.209 to 0.052 ± 0.013 (mM Trolox / g of pulp). We observed that a direct correlation exists between the amount of total phenolics and antioxidant activity in the analyzed pulps.

antioxidant activity; ABTS; DPPH and fruit


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