GENETIC VARIABILITY OF Fusarium solani AND Fusarium oxysporum f. sp. passifloraeISOLATED ASSOCIATED WITH PASSION FRUIT

The diversity of 37 isolates of Fusarium solani and 13 isolates of Fusarium oxysporum f. sp. passiflorae was evaluated using ISSR and RAPD molecular markers. The isolates were obtained from passion fruit plants with wilt symptoms, found in the main producing regions of the Northern of Minas Gerais and Sebastião Laranjeira - BA. Molecular fingerprinting were obtained using DNA extract from mycelium of monosporic culture and nine ISSR and RAPD primers. Amplification with ISSR and RAPD primers generated 121 and 126 bands, respectively, all of which showed polymorphism between at least two isolates. ISSR analysis showed interspecific genetic similarity index of 0.15. The intraspecific variability was large, ranging from 0.27 to 0.92 for F. solani and from 0.30 to 0.89 for F. o. f. sp. passiflorae. The RAPD analysis generated interspecific genetic similarity index of 0.11 and intraspecific variability indices ranging from 0.17 to 0.79 for F. solani and from 0.21 to 0.73 for F. o. f. sp. passiflorae. The isolates of F. oxysporumf. sp. passiflorae and F. solani were clustered into two distinct groups. The intraspecific variability within the two species was high.

ISSR; RAPD; Passiflorae edulis Sims f. flavicarpa; root diseases