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In vitro multiplication of Prunus rootstocks under low concentrations and different auxins

The experiments were carried out by testing the type of auxin (IBA, IAA and NAA) at four concentrations (0; 0.01; 0.1 and 1.0 muM) for each one of the four used rootstocks (G x N22, Mr.S 2/5, Marianna and Mirabolano). Each rootstock performed a trial with the same basic medium (MS ¾) except for Marianna, which was carried out with MS; the BAP concentrations were differents. To each medium, the different types and auxin concentrations were added to be tested. The rootstocks performed differently in relation to the type and auxin concentrations. The auxins IBA and IAA provided higher values than NAA for all the analyzed parameters. IBA or IAA provided the best responses at 0.01 mM for all the analyzed parameters of the rootstock G x N22. Marianna showed the best responses at 0.01 - 1.0 muM. Mirabolano rootstock, had no significantly response to the type and auxin concentration. NAA, did not provide good results for in vitro multiplication of Prunus rootstocks, and it was overcome as a whole by the control.

IBA; IAA; NAA; peach; tissue culture; in vitro culture


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