The main objective of this work was to develop regeneration protocol in order to produce transgenics cv. "Gala" plants. In the regeneration studies, different explant sources were evaluated (wounded leaf; leaf segments; internodes) in different culture media containing BAP and TDZ (0; 3,0; 4,0 and 5,0mg.L-1), and the effect of the darkness in the explant source, were also analised in the frequencies and number of regenerate shoots. The wounded leaf and leaf segments shown the best response. Pre-conditioning the explants source during a week in darkness conditions increased the regeneration rates and the number of shoots, decreasing the optimum level of BAP necessary to obtain the same number of shoots. BAP at 4,0 or 5,0mg.L-1 showed better responses however, higher TDZ concentrations promoted vitrification.
morfogenesis; growth regulators; BAP; TDZ
