This study is aimed to identify isoenzimatic variations in banana plants derived from buds of cultivar nanicão subjected to in vitro selection with 80 and 100 mol m-3 of NaCl. After acclimatization, plants were cultivated for 30 days in a greenhouse, under different levels of soil salinity of saturation extracts (ECe = 0.2, 7.0 and 14.0 dS m-1). Plants cultivated in soil with 14.0 dS m-1 did not resist the stress. Among the isoenzimatic systems evaluated, only peroxidase system showed activity in all the leaf samples. Plants regenerated from buds subjected to in vitro saline stress presented anodic and cathodic bands for treatments with 0.2 and 7.0 dS m-1, while plants formed from buds grown on salt free medium showed only cathodic bands. Alterations of band number and intensity could be due to somaclonal variation induced by salt stress during in vitro culture.
Musa spp; isoenzymes; peroxidase; somaclonal variation
