The objective of this paper was to evaluate the effect of dicamba and picloram on the induction of somatic embryogenesis in mature zygotic embryos, cotyledons and hypocotyls of Eucalyptus grandis. Callus induction was obtained in MS culture medium with dicamba (0.25; 0.5; 1.0 and 2.0 mg L-1) or picloram (0.5; 1.5; 5.0 and 10.0 mg L-1). Treatments of cotyledonary explants with dicamba (0.5 mg L-1) or picloram (5.0 and 10.0 mg L-1) resulted in structures similar to somatic embryos, which appeared in different development stages. The histological analysis confirmed the presence of independent structures with an independent vascular system, suggesting that the structures are somatic embryos.
Micropropagation; tissue culture; clonal propagation
