Imbuia has recalcitrant seeds with strong tegumental dormancy, irregular and low germination, which impair natural propagation. The objective of this study was to evaluate the in vitro germination of imbuia embryonic axes by testing the effect of different concentrations of sucrose, activated charcoal and salt formulations in order to accelerate the production of plants. Zygotic embryonic axes were inoculated in MS/2 culture medium with different concentrations of sucrose (15, 30, 60 or 90 g L-1), of activated charcoal (0, 1, 2 or 3 gL-1) and in different salt formulations (MS, WPM, MS/2 or WPM/2). The results indicated that the addition of 15 g L-1 sucrose was not enough to promote germination of embryonic axes and the other concentrations promoted between 45.5 and 59.4% germination. The best results were observed with MS and MS/2 salt formulations obtained 67.7 and 74.2% of germination respectively. The addition of activated charcoal to the media favored the in vitro germination of zygotic embryonic axes, while oxidation of the embryo tissues occurred in media without activated charcoal and germination did not happen. The in vitro germination of imbuia can be achieved with the MS/2 salt formulation supplemented with 30 g L-1 sucrose and 1 g L-1 of activated charcoal.
Sucrose; Culture medium; Activated charcoal
