This work aimed to evaluate methods of microbiolization and interaction between isolates of rhizobacteria and eucalyptus clones. We have tested the following methods of microbiolization: a) Addition of suspension of rhizobacteria (10(8) cfu/mL) to the substrate (0.2 mL/cc); b) Immersion of mini-cuttings in the inoculum suspension (10(8) cfu/mL); and c) Combination of the two methods (a and b). Mini-cuttings of a hybrid clone of eucalyptus (Eucalyptus grandis x Eucalyptus urophylla) and three (3918, FL2 and S1) rhizobacteria isolates were used. Twenty-five days after the mini-cuttings were planted, the rooting, root biomass and disease incidence were evaluated. In general, there were no significant differences between the microbiolization methods. All rhizobacterial isolates increased rooting, biomass and biocontrol of Cylindrocladium spp. In another experiment, the results showed interaction between eucalyptus clones and rhizobacteria isolates for the rooting index and root biomass.
Clonal propagation; rooting; biocontrol; PGPR
