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In vitro multiplication of hybrid clones of Eucalyptus globulus

The present study aimed to evaluate the response of hybrid Eucalyptus globulus clones in MS and JADS culture media during the in vitro multiplication phase. The explants were originated from the in vitro establishment phase of 21 Eucalyptus urophylla x E. globulus clones, being 11 clones with three in vitro introductions, and of six Eucalyptus grandis x E. globulus clones, being two clones with three introductions. The clones were subcultivated monthly in MS and JADS culture media, with 0.5 mg L-1 of BAP (6-benzylaminopurine) and 0.01 mg L-1 of NAA (naphthaleneacetic acid). It was concluded that: the MS culture medium was superior in terms of the multiplication rate; most of the clones were established in the in vitro multiplication phase, whereas some clones appeared recalcitrant in this phase of the micropropagation; the multiplication rate presented a tendency to increase in the first subcultures with a subsequent fall for most of the clones.

Culture medium; In vitro propagation; Micropropagation


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