USO DE AGENTES COMPLEXANTES PARA O AUMENTO NA PRODUÇÃO DIRECIONADA DE CICLODEXTRINAS POR ENZIMA COMERCIAL TORUZYME<sup>®</sup>

Aguiar, Maria F. A.; Fenelon, Vanderson C.; Miyoshi, Juliane H.; Valério, Nathalia M.; Mangolim, Camila S.; Matioli, Graciette

doi:10.21577/0100-4042.20230105

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Artigo • Quím. Nova 47 (3) • 2024 • https://doi.org/10.21577/0100-4042.20230105 copiar

USO DE AGENTES COMPLEXANTES PARA O AUMENTO NA PRODUÇÃO DIRECIONADA DE CICLODEXTRINAS POR ENZIMA COMERCIAL TORUZYME^®

USE OF COMPLEXING AGENTS FOR INCREASED TARGETED PRODUCTION OF CYCLODEXTRINS BY COMMERCIAL ENZYME TORUZYME^®.

Autoria SCIMAGO INSTITUTIONS RANKINGS

Cyclodextrins (CDs) are cyclic oligosaccharides, products of the intramolecular transglycosylation reaction promoted by the enzyme cyclomaltodextrin glucanotransferase (CGTase). The most commonly produced CDs are α-CD, β-CD and γ-CD, composed of 6, 7 and 8 glucose molecules, respectively. They form non-covalent inclusion complexes with several molecules, increasing their stability and/or solubility. Due to the importance of CDs and the various industrial applications, this work aimed to use the commercial enzyme Toruzyme^® to obtain CDs and direct its production to α-CD or β-CD through the use of ethanol and complexing agents in the reaction medium. The reaction medium added with ethanol and cyclohexanecarboxylic acid directed the production to β-CD, reaching 47.09 mg mL^-1 of this molecule. The production medium containing ethanol and stearic acid proved to be more advantageous for the production of α-CD and, after 48 h of reaction, the production of α-CD reached 19.57 mg mL^-1, a value six times greater than that of amount of β-CD produced. With this study it was possible to prove that with the use of ethanol and complexing agents in the reaction medium it is possible to direct the production of a certain CD and, thus, make different CGTases economically viable for industrial use.

Keywords:
complexing agents; cyclohexanecarboxylic acid; stearic acid.

Tempo de Produção (h)	Ausência de etanol 10%	Presença de etanol 10%	Presença de etanol 10% e ácido mirístico	Presença de etanol 10% e ácido esteárico
0	0	0	0	0
3	2,428^{b; A}	4,309^{a; A}	2,552^{b; A}	0,704^{c; B}
6	1,275^{b; B}	3,300^{a; B}	1,558^{b; B}	0,438^{c; C}
12	0,511^{c; BC}	1,919^{a; C}	1,131^{b; C}	1,047^{b; A}
24	0,305^{c; C}	0,915^{a; D}	0,745^{b; D}	0,762^{b; B}
36	0,177^{b; C}	0,580^{a; E}	0,507^{a; DE}	0,508^{a; C}
48	0,125^{b; C}	0,384^{a; EF}	0,436^{a; EF}	0,407^{a; CD}
60	0,118^{b; C}	0,277^{a; FG}	0,289^{a; EFG}	0,257^{a; DE}
72	0,079^{b; C}	0,240^{a; FG}	0,193^{a; FG}	0,180^{a; EF}
96	0,052^{b; C}	0,162^{a; FG}	0,136^{a; G}	0,127^{a; EF}
120	0,040^{b; C}	0,117^{a; G}	0,106^{a; G}	0,101^{a; EF}
144	0,031ª^{; C}	0,087^{a; G}	0,085^{a; G}	0,074^{a; F}
168	0,019^{a; C}	0,073^{a; G}	0,064^{a; G}	0,059^{a; F}

Tempo de Produção (h)	Ausência de etanol 10%	Presença de etanol 10%	Presença de etanol 10% e 3-fenilpropiônico	Presença de etanol 10% e ácido ciclo-hexanocarboxílico
0	0	0	0	0
3	3,245^{a; A}	2,436^{b; A}	2,630^{b; A}	1,538^{c; A}
6	2,300^{a; B}	1,718^{c; B}	2,122^{b; B}	0,944^{d; B}
12	1,384^{a; C}	1,085^{c; C}	1,230^{b; C}	0,741^{d; C}
24	0,740^{a; D}	0,641^{b; D}	0,740^{a; D}	0,439^{c; E}
36	0,490^{b; E}	0,481^{b; E}	0,590^{a; E}	0,365^{c; F}
48	0,346^{d; EF}	0,385^{c; F}	0,527^{a; E}	0,445^{b; E}
60	0,229^{d; FG}	0,299^{c; FG}	0,443^{b; F}	0,500^{a; D}
72	0,192^{d; FG}	0,249^{c; GH}	0,386^{b; F}	0,507^{a; D}
96	0,142^{d; G}	0,178^{c; HI}	0,299^{b; G}	0,419^{a; E}
120	0,107^{d; G}	0,137^{c; I}	0,210^{b; H}	0,336^{a; FG}
144	0,082^{d; G}	0,109^{c; I}	0,181^{b; H}	0,316^{a; G}
168	0,070^{d; G}	0,099^{c; I}	0,157^{b; H}	0,280^{a; H}

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USO DE AGENTES COMPLEXANTES PARA O AUMENTO NA PRODUÇÃO DIRECIONADA DE CICLODEXTRINAS POR ENZIMA COMERCIAL TORUZYME®

USE OF COMPLEXING AGENTS FOR INCREASED TARGETED PRODUCTION OF CYCLODEXTRINS BY COMMERCIAL ENZYME TORUZYME®.

USO DE AGENTES COMPLEXANTES PARA O AUMENTO NA PRODUÇÃO DIRECIONADA DE CICLODEXTRINAS POR ENZIMA COMERCIAL TORUZYME^®

USE OF COMPLEXING AGENTS FOR INCREASED TARGETED PRODUCTION OF CYCLODEXTRINS BY COMMERCIAL ENZYME TORUZYME^®.