This study aimed to analyze the physicochemical characteristics of honey and pollen of two different species of stingless bees Melipona flavolineata and Melipona fasciculata from different regions in the State of Pará, the two totally different localities, Mosqueiro Island and Castanhal municipality, different climate and vegetation directly influence the quality and characteristics of these raw materials. The honey and pollen samples were collected and stored in hermetically sealed pots, the honey was kept at room temperature and the pollen at a freezing temperature of -12 °C to -15 °C, the analyzes were carried out in the laboratory of the Universidade do Estado do Pará - Campus Castanhal. For physicochemical characterization of honey, moisture, acidity, pH, ash, insoluble solids, HMF reducing sugar, total and apparent sucrose, HMF and color were performed. For the physicochemical characterization of the pollen, moisture, ash, protein, acidity, reducing and total sugars were analyzed. Regarding the honey collected in the meliponario of the municipality of Castanhal, they obtained different results from the honey collected in Mosqueiro, even being of the same species. In the pollen collected from the stingless bees of the meliponario located on the island of Mosqueiro, there were high levels of lipids, the results of proteins obtained from the pollen of Castanhal were high, unlike the pollen of the island of Mosqueiro.