Métodos analíticos para determinar lactonas macrocíclicas en diferentes tipos de matrices. una revisión

ANALYTICAL METHODS FOR DETERMINATION OF MACROCYCLIC LACTONES IN DIFFERENT TYPES OF MATRICES. A REVIEW. The macrocyclic lactones (ML) are drugs widely used to combat endo- and ectoparasites in livestock, pets, crops, etc. Since marketing of ivermectin, the number of available ML increased considerably and, consequently, a wide variety of analytical methodologies for determination of MLs in multiple types of matrices have been published. Most of these methodologies have already been reviewed before so this paper is about those most recent and/or those have not been previously considered. In this review the focus is on biological, alimentary, and environmental matrices emphasizing on the most important techniques employed on sample preparation. Also, the liquid chromatography with of fluorescence and mass spectrometry detectors is discussed as the most used instrumental techniques for identification of MLs. Finally, a brief review is made on the importance of method validation and some strategies for its implementation.

Keywords:
macrocyclic lactones; avermectins; milbemycins; sample pretreatment; HPLC-FLD; LC/MS