Accelerating Wound Healing of Traumatic Ulcer with Topical Application of Dental Pulp Mesenchymal Stem Cell Secretome and Robusta Green Coffee Bean Extract Combination in vivo

ABSTRACT

Objective:

To investigate the ability of a combination dental pulp mesenchymal stem cell secretome (DPMSCS), robusta green coffee bean extract (RGCBE), and Carboxymethylcellulose-Natrium (CMC-Na) in a Wistar rats model of traumatic ulcers.

Material and Methods:

Twenty-eight young, male, healthy Wistar rats (Rattus norvegicus) were divided into seven groups randomly: Group K₀, group K₁-3 (traumatic ulcer rats that received CMC-Na gel for three days), group K₁-7 (traumatic ulcer rats that received CMC-Na gel for seven days), group K₂-3 (traumatic ulcer rats that received RGCBE for three days), group K₂-7 (traumatic ulcer rats that received RGCBE for seven days), group K₃-3 (traumatic ulcer rats that received DPMSCS for three days), and group K₃-7 (traumatic ulcer rats that received DPMSCS for seven days). An ulcer was made with an amalgam stopper on the right buccal mucosa of the rats. DPMSCS 50% gel was applied to the ulcer on the left buccal mucosa. The ulcer diameter was measured on day 3 and day 7.

Results:

There was a significant difference in the diameter of the ulcer, the number of neutrophils, and fibroblasts in the treatment group compared to the control group on day 7.

Conclusion:

A combination of DPMSCS and RGCBE 50% accelerates traumatic ulcer wound healing by lowering ulcer diameter, decreasing neutrophil counts, and increasing fibroblast proliferation in vivo.

Keywords:
Fibroblasts; Neutrophils; Stem Cells; Wound Healing; Medicine