<i>In vitro</i> growth of <i>Cattleya nobilior</i> Rchb. f.: culture media, sealing systems and irradiance

Freitas, Kaliana Gottschalk de; Sorgato, José Carlos; Soares, Jackeline Schultz; Ribeiro, Luan Marlon

doi:10.1590/1983-40632021v5167131

Kaliana Gottschalk de Freitas

Universidade Federal da Grande Dourados, Faculdade de Ciências Agrárias, Dourados, MS, Brasil. E-mail/ORCID: kalianadefreitas@gmail.com/0000-0003-2223-3676; josesorgato@ufgd.edu.br/0000-0003-0001-5467; jacke.schultz@gmail.com/0000-0001-9497-2975; luanmarlon@hotmail.com/0000-0002-7278-7696.

https://orcid.org/0000-0003-2223-3676

José Carlos Sorgato

Universidade Federal da Grande Dourados, Faculdade de Ciências Agrárias, Dourados, MS, Brasil. E-mail/ORCID: kalianadefreitas@gmail.com/0000-0003-2223-3676; josesorgato@ufgd.edu.br/0000-0003-0001-5467; jacke.schultz@gmail.com/0000-0001-9497-2975; luanmarlon@hotmail.com/0000-0002-7278-7696.

https://orcid.org/0000-0003-0001-5467

Jackeline Schultz Soares

Universidade Federal da Grande Dourados, Faculdade de Ciências Agrárias, Dourados, MS, Brasil. E-mail/ORCID: kalianadefreitas@gmail.com/0000-0003-2223-3676; josesorgato@ufgd.edu.br/0000-0003-0001-5467; jacke.schultz@gmail.com/0000-0001-9497-2975; luanmarlon@hotmail.com/0000-0002-7278-7696.

https://orcid.org/0000-0001-9497-2975

Luan Marlon Ribeiro

Universidade Federal da Grande Dourados, Faculdade de Ciências Agrárias, Dourados, MS, Brasil. E-mail/ORCID: kalianadefreitas@gmail.com/0000-0003-2223-3676; josesorgato@ufgd.edu.br/0000-0003-0001-5467; jacke.schultz@gmail.com/0000-0001-9497-2975; luanmarlon@hotmail.com/0000-0002-7278-7696.

https://orcid.org/0000-0002-7278-7696

VF	DF	-------------------------------------------------------------------- Mean square --------------------------------------------------------------------
		PH	PD	RL	LL	FW	NL	NR	NS
		------------------------------------- mm -------------------------------------				g	NL	NR	NS
I	1	31.05^ns ns not significant by the Tukey test at 5 % of probability. VF: variation factor; DF: degrees of freedom; OM: overall mean; CM: culture medium; SS: sealing system; I: irradiance.	0.03^ns ns not significant by the Tukey test at 5 % of probability. VF: variation factor; DF: degrees of freedom; OM: overall mean; CM: culture medium; SS: sealing system; I: irradiance.	0.70^ns ns not significant by the Tukey test at 5 % of probability. VF: variation factor; DF: degrees of freedom; OM: overall mean; CM: culture medium; SS: sealing system; I: irradiance.	389.62^* * Significant;	0.05^ns ns not significant by the Tukey test at 5 % of probability. VF: variation factor; DF: degrees of freedom; OM: overall mean; CM: culture medium; SS: sealing system; I: irradiance.	50.12^* * Significant;	50.12^ns ns not significant by the Tukey test at 5 % of probability. VF: variation factor; DF: degrees of freedom; OM: overall mean; CM: culture medium; SS: sealing system; I: irradiance.	3.48^* * Significant;
CM	4	319.88^* * Significant;	1.37^ns ns not significant by the Tukey test at 5 % of probability. VF: variation factor; DF: degrees of freedom; OM: overall mean; CM: culture medium; SS: sealing system; I: irradiance.	99.51^* * Significant;	103.00^ns ns not significant by the Tukey test at 5 % of probability. VF: variation factor; DF: degrees of freedom; OM: overall mean; CM: culture medium; SS: sealing system; I: irradiance.	0.99^* * Significant;	63.20^ns ns not significant by the Tukey test at 5 % of probability. VF: variation factor; DF: degrees of freedom; OM: overall mean; CM: culture medium; SS: sealing system; I: irradiance.	63.20^ns ns not significant by the Tukey test at 5 % of probability. VF: variation factor; DF: degrees of freedom; OM: overall mean; CM: culture medium; SS: sealing system; I: irradiance.	1.70^ns ns not significant by the Tukey test at 5 % of probability. VF: variation factor; DF: degrees of freedom; OM: overall mean; CM: culture medium; SS: sealing system; I: irradiance.
SS	1	1,190.32^* * Significant;	3.49^* * Significant;	33.20^ns ns not significant by the Tukey test at 5 % of probability. VF: variation factor; DF: degrees of freedom; OM: overall mean; CM: culture medium; SS: sealing system; I: irradiance.	1,882.82^* * Significant;	0.55^ns ns not significant by the Tukey test at 5 % of probability. VF: variation factor; DF: degrees of freedom; OM: overall mean; CM: culture medium; SS: sealing system; I: irradiance.	135.57^* * Significant;	135.57^* * Significant;	10.53^* * Significant;
I x CM	4	198.05^* * Significant;	3.76^* * Significant;	82.53^* * Significant;	72.02^ns ns not significant by the Tukey test at 5 % of probability. VF: variation factor; DF: degrees of freedom; OM: overall mean; CM: culture medium; SS: sealing system; I: irradiance.	1.84^* * Significant;	163.80^* * Significant;	163.80^* * Significant;	0.52^ns ns not significant by the Tukey test at 5 % of probability. VF: variation factor; DF: degrees of freedom; OM: overall mean; CM: culture medium; SS: sealing system; I: irradiance.
I x SS	1	19.97^ns ns not significant by the Tukey test at 5 % of probability. VF: variation factor; DF: degrees of freedom; OM: overall mean; CM: culture medium; SS: sealing system; I: irradiance.	4.45^* * Significant;	0.99^ns ns not significant by the Tukey test at 5 % of probability. VF: variation factor; DF: degrees of freedom; OM: overall mean; CM: culture medium; SS: sealing system; I: irradiance.	68.90^ns ns not significant by the Tukey test at 5 % of probability. VF: variation factor; DF: degrees of freedom; OM: overall mean; CM: culture medium; SS: sealing system; I: irradiance.	1.44^* * Significant;	16.46^ns ns not significant by the Tukey test at 5 % of probability. VF: variation factor; DF: degrees of freedom; OM: overall mean; CM: culture medium; SS: sealing system; I: irradiance.	16.46^ns ns not significant by the Tukey test at 5 % of probability. VF: variation factor; DF: degrees of freedom; OM: overall mean; CM: culture medium; SS: sealing system; I: irradiance.	0.10^ns ns not significant by the Tukey test at 5 % of probability. VF: variation factor; DF: degrees of freedom; OM: overall mean; CM: culture medium; SS: sealing system; I: irradiance.
CM x SS	4	307.57^* * Significant;	2.52^* * Significant;	36.60^ns ns not significant by the Tukey test at 5 % of probability. VF: variation factor; DF: degrees of freedom; OM: overall mean; CM: culture medium; SS: sealing system; I: irradiance.	133.40^ns ns not significant by the Tukey test at 5 % of probability. VF: variation factor; DF: degrees of freedom; OM: overall mean; CM: culture medium; SS: sealing system; I: irradiance.	1.18^* * Significant;	20.86^ns ns not significant by the Tukey test at 5 % of probability. VF: variation factor; DF: degrees of freedom; OM: overall mean; CM: culture medium; SS: sealing system; I: irradiance.	20.86^ns ns not significant by the Tukey test at 5 % of probability. VF: variation factor; DF: degrees of freedom; OM: overall mean; CM: culture medium; SS: sealing system; I: irradiance.	1.57^ns ns not significant by the Tukey test at 5 % of probability. VF: variation factor; DF: degrees of freedom; OM: overall mean; CM: culture medium; SS: sealing system; I: irradiance.
I x CM x SS	4	293.36^* * Significant;	2.74^* * Significant;	21.39^* * Significant;	313.05^* * Significant;	1.16^* * Significant;	17.88^ns ns not significant by the Tukey test at 5 % of probability. VF: variation factor; DF: degrees of freedom; OM: overall mean; CM: culture medium; SS: sealing system; I: irradiance.	17.88^ns ns not significant by the Tukey test at 5 % of probability. VF: variation factor; DF: degrees of freedom; OM: overall mean; CM: culture medium; SS: sealing system; I: irradiance.	1.20^ns ns not significant by the Tukey test at 5 % of probability. VF: variation factor; DF: degrees of freedom; OM: overall mean; CM: culture medium; SS: sealing system; I: irradiance.
Total		12,511.58	114.74	37,683.91	11,218.50	52.32	4,045.76	2,598.55	153.87
OM		24.02	2.37	42.18	18.16	0.92	8.65	9.17	2.14
CV (%)		16.04	12.91	17.48	19.25	16.42	25.60	20.17	20.20

Plant height
Medium	----------------------- Natural ventilation -----------------------		----------------------- Conventional ventilation -----------------------
Medium	3,000 K-1 LED	3,000 K-2 LED	3,000 K-1 LED	3,000 K-2 LED
B100	32.54 abA^a^* * Lowercase letters in the column compare the culture media for the same sealing system and irradiance, while uppercase letters in the row compare the irradiance for the same culture medium and sealing system. Overwritten letters in the row compare the sealing system for the same culture medium and irradiance (Tukey; p < 0.05). B100: 100 g of banana pulp; B200: 200 g of banana pulp; C100: 100 g of banana peel; C200: 200 g of banana peel; MS: Murashige & Skoog (1962); 3,000 K-1 LED: 43 µmol m-2 s-1; 3,000 K-2 LED: 86 µmol m-2 s-1.	38.79 aA^a	20.27 aA^b	14.16 bA^b
B200	30.61 abA^a	27.30 abcA^a	23.04 aB^a	33.02 aA^a
C100	34.57 aA^a	16.51 cB^a	22.50 aA^b	18.40 bA^a
C200	21.93 abA^a	30.47 abA^a	23.29 aA^a	17.29 bA^b
MS	20.80 bA^a	18.20 bcA^a	15.77 aA^a	21.00 abA^a
Mean	28.09	26.21	20.97	20.97
CV (%)	16.04

Pseudobulb diameter
Medium	----------------------- Natural ventilation -----------------------		----------------------- Conventional ventilation -----------------------
Medium	3,000 K-1 LED	3,000 K-2 LED	3,000 K-1 LED	3,000 K-2 LED
B100	2.83 aA^a^* * Lowercase letters in the column compare the culture media for the same sealing system and irradiance, while uppercase letters in the row compare the irradiance for the same culture medium and sealing system. Overwritten letters in the row compare the sealing system for the same culture medium and irradiance (Tukey; p < 0.05). B100: 100 g of banana pulp; B200: 200 g of banana pulp; C100: 100 g of banana peel; C200: 200 g of banana peel; MS: Murashige & Skoog (1962); 3,000 K-1 LED: 43 µmol m-2 s-1; 3,000 K-2 LED: 86 µmol m-2 s-1.	2.76 aA^a	2.20 aA^a	0.56 cB^b
B200	2.12 aB^a	3.14 aA^a	1.95 aB^a	2.93 bcA^a
C100	2.56 aA^a	2.20 aA^a	2.54 aA^a	1.60 abB^a
C200	2.08 aB^b	3.41 aA^a	3.24 aA^a	2.01 aB^b
MS	2.11 aA^a	2.25 aA^a	1.99 aB^a	3.06 aA^a
Mean	2.34	2.75	2.38	2.03
CV (%)	12.91

Length of the largest root
Medium	----------------------- Natural ventilation -----------------------		----------------------- Conventional ventilation -----------------------
Medium	3,000 K-1 LED	3,000 K-2 LED	3,000 K-1 LED	3,000 K-2 LED
B100	13.33 aA^a^* * Lowercase letters in the column compare the culture media for the same sealing system and irradiance, while uppercase letters in the row compare the irradiance for the same culture medium and sealing system. Overwritten letters in the row compare the sealing system for the same culture medium and irradiance (Tukey; p < 0.05). B100: 100 g of banana pulp; B200: 200 g of banana pulp; C100: 100 g of banana peel; C200: 200 g of banana peel; MS: Murashige & Skoog (1962); 3,000 K-1 LED: 43 µmol m-2 s-1; 3,000 K-2 LED: 86 µmol m-2 s-1.	6.16 bB^a	12.33 aA^a	8.50 abA^a
B200	7.83 abcA^a	10.00 abA^a	11.66 aA^a	14.50 aA^a
C100	10.83 abA^a	9.16 abA^a	9.50 aA^a	10.50 abA^a
C200	4.00 cA^a	4.83 bA^a	8.16 aA^a	6.00 bA^a
MS	6.66 bcB^a	13.65 aA^a	7.16 aA^a	8.66 abA^b
Mean	8.53	8.76	9.76	9.63
CV (%)	17.48

Leaf length
Medium	----------------------- Natural ventilation -----------------------		----------------------- Conventional ventilation -----------------------
Medium	3,000 K-1 LED	3,000 K-2 LED	3,000 K-1 LED	3,000 K-2 LED
B100	27.05 aA^a^* * Lowercase letters in the column compare the culture media for the same sealing system and irradiance, while uppercase letters in the row compare the irradiance for the same culture medium and sealing system. Overwritten letters in the row compare the sealing system for the same culture medium and irradiance (Tukey; p < 0.05). B100: 100 g of banana pulp; B200: 200 g of banana pulp; C100: 100 g of banana peel; C200: 200 g of banana peel; MS: Murashige & Skoog (1962); 3,000 K-1 LED: 43 µmol m-2 s-1; 3,000 K-2 LED: 86 µmol m-2 s-1.	24.4 abA^a	11.85 abA^b	7.90 aA^b
B200	24.67 aA^a	22.30 abcA^a	16.42 abA^a	17.50 aA^a
C100	29.63 aA^a	12.12 cB^a	14.84 abA^b	14.35 aA^a
C200	21.06 aA^a	26.32 aA^a	22.95 aA^a	9.35 aB^b
MS	20.17 aA^a	12.68 bcA^a	10.18 bA^b	16.70 aA^a
Mean	24.51	19.56	15.25	13.16
CV (%)	19.25

Fresh weight
Medium	----------------------- Natural ventilation -----------------------		----------------------- Conventional ventilation -----------------------
Medium	3,000 K-1 LED	3,000 K-2 LED	3,000 K-1 LED	3,000 K-2 LED
B100	1.66 aA^a^* * Lowercase letters in the column compare the culture media for the same sealing system and irradiance, while uppercase letters in the row compare the irradiance for the same culture medium and sealing system. Overwritten letters in the row compare the sealing system for the same culture medium and irradiance (Tukey; p < 0.05). B100: 100 g of banana pulp; B200: 200 g of banana pulp; C100: 100 g of banana peel; C200: 200 g of banana peel; MS: Murashige & Skoog (1962); 3,000 K-1 LED: 43 µmol m-2 s-1; 3,000 K-2 LED: 86 µmol m-2 s-1.	0.93 aB^a	0.77 aA^b	0.38 bA^a
B200	0.95 abA^a	1.02 aA^b	0.70 aB^a	2.30 aA^a
C100	1.52 aA^a	0.62 aB^a	0.78 aA^b	0.77 bA^a
C200	0.58 bA^a	0.99 aA^a	0.81 aA^a	0.60 bA^a
MS	0.59 bA^a	1.00 aA^a	0.53 aA^a	0.84 bA^b
Mean	1.06	0.91	0.72	0.98
CV (%)

Sealing system	NL	NP
NVS	7.59 b^* * Means followed by the same letter in each variable do not differ by the Tukey test (p < 0.05). NVS = natural ventilation system; CVS = conventional ventilation system.	1.84 b
CVS	9.72 a	2.43 a
Mean	8.65	2.13
CV (%)	25.60	20.20

[TFN1] ^*
Significant;

[TFN2] ^ns
not significant by the Tukey test at 5 % of probability. VF: variation factor; DF: degrees of freedom; OM: overall mean; CM: culture medium; SS: sealing system; I: irradiance.

Brasil

Brasil

In vitro growth of Cattleya nobilior Rchb. f.: culture media, sealing systems and irradiance

Crescimento in vitro de Cattleya nobilior Rchb. f.: meios de cultura, sistemas de vedação e irradiância

ABSTRACT