The objective of this work was to validate the association of single nucleotide polymorphism (SNP) markers for the genes FAD3A, FAD3B, and FAD3C with the linolenic acid content (18:3) in soybean seeds and to analyze the influence of the genetic parameters of these markers on this trait. One hundred and eighty‑five F2 progenies derived from a cross between A29 (mutant for the three FAD3 genes, 1% 18:3 content) and Tucunaré (wild‑type genotype, 11% 18:3 content) were genotyped. The molecular markers for genes FAD3A, FAD3B, and FAD3C explained the variations in 18:3 content of the F2 and F2:3 segregating populations. In addition, allelic substitutions in the FAD3A locus provide greater variations in 18:3 content than substitutions in the other two loci.
Glycine max; FAD3A; FAD3B; FAD3C; 18:3; allelic replacement
