Influence of pulsing and wet cold storage on the vase water microbial profiles and overall quality of cut gladioli

Chore, Judith Kavulani; Mwangi, Mariam; Mbuthia, Stephen Karori; Sibiyia, Lynet Ongachi

doi:10.1590/2447-536X.v27i4.2328

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Scientific Article • Ornam. Hortic. 27 (4) • Oct-Dec 2021 • https://doi.org/10.1590/2447-536X.v27i4.2328 copy

Influence of pulsing and wet cold storage on the vase water microbial profiles and overall quality of cut gladioli

Influência do pulsing e armazenamento úmido sobre os perfis microbianos e qualidade geral de gladíolo de corte

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

Occlusion of the stem vasculature by microorganisms that proliferate in the vase water, or the plant vessels, leads to water stress symptoms that reduce postharvest quality of cut flowers. This study aimed to determine the effects of pulsing and wet-cold storage on the microbial profiles in cut Gladiolus grandiflorus L. cv. Fado. Pulsing treatments of 600-ppm 8-hydroxyquinoline sulfate plus 5% sucrose solution versus distilled water were administered before wet cold storage periods of 0-5 days in cut Gladiolus, previously grown from corms under open field. A two-by-six factorial experiment embedded in a completely randomized design with four replicates was accomplished. Proc GLM in two-way Anova was adopted, and the means were separated using Tukey’s test at a 5% level of significance. The pulsing treatment of 600 ppm 8-HQS plus 5% sucrose, the wet cold storage duration and their interactive effects significantly (P˂0.0209; ˂0.0001 and ˂0.0001 respectively) affected the means of the colony-forming units in the vase water of cut Gladiolus at senescence. The prolonged vase life of cut gladioli spikes was associated with decreased microbial proliferation as influenced by pulsing and wet storage duration of up to 4 days. Data generated from this study will improve existing technologies related to the quality and market value of this Gladiolus cultivar.

Keywords:
Gladiolus grandiflorus; vase; 8-hydroxyquinoline; microbiota

Days of storage	Initial log 10 cfu mL^-1		Vase life (days)		Isolates in Gladiolus vase water
Days of storage	Non-pulsed	Pulsed	Non-pulsed	Pulsed	Non-pulsed	Pulsed
0	2.228e	1.935g	4.75^c	8.75^b	Aeromonas hydrophila	Proteus vulgaris
1	2.215e	1.833h	9.50^ab	10.25^ab	Bacillus species	Serratia marscescens
2	2.178ef	1.925g	9.50^ab	10.00^ab	Hafnia alvei	Staphylococcus aureus
3	1.253i	2.102f	9.75^ab	11.50^a	Pantoea agglomerans	Pantoea agglomerans Proteus vulgaries Staphylococcus species
4	2.46c	2.336d	11.00^ab	11.25^ab	Bacillus species	Bacillus species
5	2.734b	3.264a	10.25^hi	10.25^ab	Fusarium	Staphylococcus aureus
Means	2.178a	2.232b	9.13^a	10.33^b
CD at 5 %	0.007	0.007	0.21	0.21
Pulsing (P)	<0.0001*		0.003*
Period of Storage	<0.0001*		<.0001*
Treatment × Storage	<0.0001*		0.0041 ⃰

Days of storage	Third day in vase log 10 cfu mL^-1		Vase Life (days)		Isolates in Gladiolus vase water
Days of storage	Non-pulsed	Pulsed	Non- pulsed	Pulsed	Non-pulsed	Pulsed
0	3.387bcd	3.208cd	4.75^c	8.75^b	Penicillium species Shewanella putrefaciens Salmonella species Vibrionaceae Aeromonas hydrophila	Pseudomonas putida Pantoea agglomerans Bacillus species Aspergillus species Ulocladium Staphylococcus aureus
1	2.584ef	3.539bc	9.50^ab	10.25^ab	Penicillium species Bacillus species Serratia marscescens	Aspergillus fumigatus Serratia marscescens Pantoea agglomerans
2	2.359ef	2.215f	9.50^ab	10.00^ab	Hafnia alvei	Albugo species Aspergillus species Staphylococcus aureus
3	4.696a	3.251bcd	9.75^ab	11.50^a	Alternaria alternata Pantoea agglomerans	Acremonium species Paecilomyces Phytophthora Alternaria species
4	3.696b	2.637e	11.00^ab	11.25^ab	Bacillus species Candida albicans Uromyces species	Bacillus species Cladosporium
5	3.097d	4.720a	10.25^hi	10.25^ab	Fusarium Exophiala	Madurella Tricothecium roseum Exophiala jeanselmei Staphylococcus
Means	3.288a	3.261a	9.13^a	10.33^b
CD at 5%	2.028	2.028	0.21	0.21
Pulsing (P)	0.5453		0.003*
Period of Storage	<0.0001*		<0.0001*
Treatment × Storage	<0.0001*		0.0041*

Days of storage	Senescence log 10 cfu mL^-1		Vase Life (days)		Isolates in Gladiolus vase water
Days of storage	Non-pulsed	Pulsed	Non-pulsed	Pulsed	Non-pulsed	Pulsed
0	6.738abc	6.093bcde	4.75^c	8.75^b	Aspergillus niger Penicillium species Cladosporium oxysporum Altanaria altanata Aspergillus flavus	Syncephalastrum Trichophyton verrucosum Epidermophyton Nigrospora E. nidulans, Acremonium species Ulocladium
1	5.579def	7.255a	9.50^ab	10.25^ab	Penicillium species Aspergillus niger Alternaria species Aspergillus flavus Acremonium species	Aspergillus fumigatus Rhizopus species Talaromyces Aspergillus parasiticus
2	6.784abc	6.402abcd	9.50^ab	10.00^ab	Alternaria alternate Aspergillus niger Alternaria species Aspergillus flavus Acremonium species	Albugo species Aspergillus species Alternaria species
3	7.103ab	5.331ef	9.75^ab	11.50^a	Alternaria species Aspergillus nidulans Acremonium species	Alternaria species, Acremonium species Phytophthora Paecilomyces species
4	5.914cdef	4.973f	11.00^ab	11.25^ab	Candida albicans Acremonium species Aspergillus niger Aspergillus flavus Uromyces	Rhizopus Aspergillus nidulans Aspergillus flavus Cladosporium species
5	5.258ef	5.506def	10.25^hi	10.25^ab	Cephalotricum Fusarium Exophiala jeanselmei Candida albicans Tricothecium	Tricothecium roseum Exophiala jeanselmei Zygomycete Lichhen Madurella species
Means	6.229a	5.926b	9.13^a	10.33^b
CD at 5 %	3.008	3.008	0.21	0.21
Pulsing (P)	0.0209*		0.003
Period of Storage	<0.0001*		<0.0001*
Treatment * Storage	<0.0001*		0.0041*

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[1] * Corresponding author: jchore@egerton.ac.ke