Features of eggshell morphology in Anastrepha sp.2 aff. fraterculus were evaluated in mature ovariole ova dissected from females preserved in ethanol, and from ovaries of fresh killed females in comparison to that of freshly laid eggs. The characteristics of sculpturing as well as of internal structure of the chorion in oviposited eggs could be observed in both samples of mature ovariole ova. Ethanol preserved females may be used as a source of eggs for morphological studies allowing this kind of analysis to species whose eggs are not readily obtained.
Chorion; vitelline membrane; ovary
