Protein and amino acid composition of wild caught freshwater crayfish (<i>Pontastacus leptodactylus</i>) in the reproductive season

Harlıoğlu, Ayşe Gül; Yılmaz, Ökkeş; Erdoğdu, Ayşe; Sıltar, Yeşim Bulut

doi:10.1590/2358-2936e2021044

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Original Article • Nauplius 29 • 2021 • https://doi.org/10.1590/2358-2936e2021044 copy

Protein and amino acid composition of wild caught freshwater crayfish (Pontastacus leptodactylus) in the reproductive season

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

Interest in feeding crayfish under culture conditions has increased in the last few years; however, there is still a lack of information on feeding crayfish according to their nutritional requirements (i.e., protein and amino acid dietary demands of broodstock). In this study, the protein and amino acid composition of abdominal muscle, gonads and hepatopancreas of male and female broodstock crayfish Pontastacus leptodactylus (Eschscholtz, 1823) were determined in the reproductive season. The results show that in P. leptodactylus, the amount of protein in the ovary was 47.25 mg/g and that it was 35.03 mg/g and 39.36 mg/g in the testes and vasa deferentia, respectively. In males, the values of essential amino acids (EAA) obtained in the abdominal muscle were significantly lower than those obtained from the hepatopancreas, testes and vasa deferentia (P < 0.05). In female crayfish, EAA/total amino acids (TAA) were found to be 45.48 % in the ovary, which is significantly higher than that of the abdominal muscle (40.19 %) and hepatopancreas (42.14 %) (P < 0.05). The results also show that abdominal valine, threonine, lysine, and histidine were statistically higher in female crayfish than male crayfish (P < 0.01). Leucine was the major EAA found in males in abdominal muscle (8.73 %). In conclusion, this study analyzed the protein and amino acid composition of abdominal muscle, hepatopancreas and gonad of crayfish in the breeding season in order to understand the protein and amino acid contents of wild caught crayfish and get an idea on the nutritional requirements of P. leptodactylus in the reproductive season.

Keywords
Crustacean metabolism; gonad; hepatopancreas; protein composition

Protein (%)	Abdomen muscle	Hepatopancreas	Ovary	Testes	Vasa deferentia
Female	40.19±2.04	36.89±2.78	47.25±2.75	-	-
Male	40.11±1.62	31.73±2.56	-	35.03±0.34	39.36±1.57
Statistical comparison of protein content	Ovary - Testes	Ovary - Vasa Deferentia	Male and female hepatopancreas	Male and female abdomen Muscle
Protein	***	***	*	ns

Amino acids (% )	Abdomen muscle	Hepatopancreas	Testes	Vasa deferentia
EAA ^†
Valine	7.44±0.32^a	6.92±0.33^bc	7.07±0.18^ab	6.60±0.25^c
Leucine	8.73±0.80^b	9.94±0.64^a	9.84±0.35^a	9.71±0.31^a
Isoleucine	4.58±0.30^b	5.10±0.28^a	5.34±0.10^a	5.14±0.08^a
Threonine	3.72±0.40^c	3.30±0.47^c	5.17±0.23^b	6.07±0.17^a
Methionine	2.53±0.79^a	1.20±0.59^b	2.22±0.16^a	1.96±0.29^a
Phenylalanine	6.35±0.69^bc	7.37±0.50^a	6.96±0.32^ab	6.09±0.18^c
Lysine	0.78±0.08^b	4.51±0.48^a	0.78±0.01^b	1.06±0.2^b
Histidine	1.19±0.25^c	1.08±0.23^c	2.28±0.35^a	1.71±0.33^b
Arginine	1.38±0.29^a	1.35±0.39^a	1.77±0.45^a	1.22±0.35^a
Tryptophan	0.12±0.04^c	0.57±0.18^a	0.31±0.04^bc	0.44±0.14^ab
ƩEAA ^†	36.85±0.31^b	41.39±1.58^a	41.80±0.61^a	40.01±1.07^a
NEAA^‡
Tyrosine	6.27±1.32^a	5.58±0.18^ab	5.56±0.44^ab	5.12±0.21^b
Alanine	8.17±1.01^a	7.20±0.63^b	7.73±0.61^a	8.69±0.32^a
Glycine	5.27±0.91^b	6.91±0.54^a	6.41±0.31^a	6.78±0.27^a
Serine	9.96±1.83^a	8.36±0.30^b	8.29±0.96^b	9.95±0.12^a
Proline	1.03±0.15^a	1.16±0.29^a	1.06±0.67^a	0.70±0.35^a
Aspartic acid	15.80±0.98^b	16.45±0.94^b	18.40±0.58^a	17.94±0.24^a
Glutamic acid	1.56±0.44^a	1.57±0.18^a	1.07±0.80^ab	0.44±0.23^b
Cysteine	9.28±0.48^a	9.31±0.70^a	9.45±0.64^a	9.93±0.42^a
ƩNEAA^‡	57.36±2.97^ab	56.56±1.18^b	58.01±0.56^ab	59.58±0.32^a
TAA^§	94.21±3.28^b	97.96±2.38^a	99.82±0.32^a	99.60±0.76^a
EAA/TAA(%)	39.14±1.05^b	42.24±0.82^a	41.88±0.57^a	40.17±0.77^b

Amino acids (% )	Abdomen muscle	Hepatopancreas	Ovary
EAA^†
Valine	6.67±0.16^a	5.68±0.49^a	7.64±0.89^a
Leucine	9.64±0.70^a	8.81±0.84^a	8.53±0.32^a
Isoleucine	4.94±0.17^a	4.24±0.43^b	4.66±0.14^ab
Threonine	5.53±0.47^b	3.67±0.98^c	8.77±0.38^a
Methionine	2.48±0.30^a	1.20±0.31^b	1.32±0.17^b
Phenylalanine	7.10±0.08^a	6.32±1.97^a	5.81±0.26^a
Lysine	0.32±0.13^c	6.83±0.72^a	3.75±0.97^b
Histidine	1.85±0.12^a	1.52±0.34^a	1.85±0.24^a
Arginine	1.40±0.86^a	0.83±0.13^a	1.33±0.26^a
Tryptophan	0.16±0.03^b	1.21±0.47^a	0.78±0.22^a
ƩEAA^†	40.12±0.76^a	40.35±4.85^a	44.47±2.01^a
NEAA^†
Tyrosine	5.55±0.85^a	5.94±1.22^a	5.96±0.68^a
Alanine	8.65±0.91^a	6.65±0.97^b	6.61±0.42^b
Glycine	6.17±1.18^a	5.37±1.38^a	5.31±0.23^a
Serine	8.44±0.79^b	8.23±0.98^b	10.57±0.88^a
Proline	0.98±0.82^a	0.67±0.26^a	1.22±0.78^a
Aspartic acid	18.25±0.32^a	15.07±0.50^b	13.42±0.41^c
Glutamic acid	1.38±0.49^a	1.46±0.37^a	0.90±0.07^a
Cysteine	10.25±0.55^a	11.69±2.50^a	9.25±0.25^a
ƩNEAA^‡	59.71±0.82^a	55.13±1.03^b	53.27±0.98^c
TAA^§	98.83±0.14^a	97.75±2.36^a	95.48±5.21^a
EAA/TAA (%)	40.19±0.78^b	42.14±2.88^b	45.48±1.14^a

Amino acids (% wet weight)	Abdomen muscle	Hepatopancreas	Ovary-Testes	Ovary-Vasa deferentia
Valine	**	**	ns	ns
Leucine	ns	ns	**	**
Isoleucine	ns	*	***	**
Threonine	**	ns	***	***
Methionine	ns	ns	***	**
Phenylalanine	ns	ns	**	ns
Lysine	**	**	***	**
Histidine	**	ns	ns	ns
Arginine	ns	*	ns	ns
Tryptophan	ns	*	**	*
ƩEAA^†	**	ns	*	**
Tyrosine	ns	ns	ns	ns
Alanine	ns	ns	*	***
Glycine	ns	ns	**	***
Serine	ns	ns	*	ns
Proline	ns	*	ns	ns
Aspartic acid	**	ns	***	***
Glutamic acid	ns	ns	ns	*
Cysteine	ns	ns	ns	*
ƩNEAA^‡	ns	ns	***	***

Sociedade Brasileira de Carcinologia Instituto de Biociências, UNESP, Campus Botucatu, Rua Professor Doutor Antônio Celso Wagner Zanin, 250 , Botucatu, SP, 18618-689 - Botucatu - SP - Brazil
E-mail: editor.nauplius@gmail.com

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[1] CORRESPONDING AUTHOR
Ayşe Gül Harlıoğlu aharlioglu@firat.edu.tr