Latex proteins downregulate inflammation and restores blood-coagulation homeostasis in acute Salmonella infection

BACKGROUND

Calotropis procera latex protein fraction (LP) was previously shown to protect animals from septic shock. Further investigations showed that LP modulate nitric oxide and cytokines levels.

OBJECTIVES

To evaluate whether the protective effects of LP, against lethal bacterial infection, is observed in its subfractions (LP_PII and LP_PIII).

METHODS

Subfractions (5 and 10 mg/kg) were tested by i.p. administration, 24 h before challenging with lethal injection (i.p.) of Salmonella Typhimurium. LP_PIII (5 mg/kg) which showed higher survival rate was assayed to evaluate bacterial clearance, histopathology, leukocyte recruitment, plasma coagulation time, cytokines and NO levels.

FINDINGS

LP_PIII protected 70% of animals of death. The animals given LP_PIII exhibited reduced bacterial load in blood and peritoneal fluid after 24 h compared to the control. LP_PIII promoted macrophage infiltration in spleen and liver. LP_PIII restored the coagulation time of infected animals, increased IL-10 and reduced NO in blood.

MAIN CONCLUSIONS

LP_PIII recruited macrophages to the target organs of bacterial infection. This addressed inflammatory stimulus seems to reduce bacterial colonisation in spleen and liver, down regulate bacterial spread and contribute to avoid septic shock.

Key words:
cytokines; coagulation; haemostasis; nitric oxide; peritoneal cavity