Seed Longevity of <i>Enterolobium contortisiliquum</i> (Vell.) Morong

Torres, Maria Fernanda Oliveira; Ferreira, Robério Anastácio; Prata, Laura Catharine Dória; Silva-Mann, Renata

doi:10.1590/2317-1545v42239618

Abstract:

Seed storage under controlled temperature and humidity maintains the viability and the vigor of seeds for a long time while preserving their longevity. Ex situ conservation in a seed storage chamber is perfect for simulating the cool dry conditions that are associated with long-term seed storage. This helps to preserve the availability of seeds for the recovery of degraded areas and to ensure a supply of propagating material for various purposes in silvicultural production, or as raw materials for biotechnological use. The present work evaluated the physical and physiological quality of Enterolobium contortisiliquum (Vell.) Morong seeds from an ex situ collection that was kept in a seed storage chamber at 6 °C ± 3 °C and with 60% ± 5% of RH in waterproof packaging. The water content, the physical quality by X-ray tests, germination, electrical conductivity, and the integrity and quality of RNA that was extracted from the embryonic axes were all determined. It was possible to identify full, damaged, malformed, translucent, and empty seeds. The seeds that had been stored for 1, 5, 9, and 10 years presented 78, 82, 42, and 38% of normal seedlings, respectively, while the lots of 6 and 7 years presented 0 and 6%. The seed storage conditions were the same for all of the lots. A water content higher than 6% influenced the quality of the seeds. The E. contortisiliquum seeds demonstrated high longevity. They could be stored for up to 10 years under controlled conditions.

Index terms:
X-ray; viability; ex situ conservation; monkfish

[1] *Corresponding Author
Email: renatamann@academico.ufs.br

Time (year)	Water content (%)	GPDL*	Category	Total (%)	Germination
Time (year)	Water content (%)	GPDL*	Category	Total (%)	NS%	AS%	D%
1	4.61	177.25 a	With Damage	4.00	3.00	0.50	0.50
			Malformed	3.00	2.50	0.50	0.00
			Full	90.00	69.00	11.00	10.00
			Translucent	3.00	2.00	0.00	0.50
			Empty	0.00	0.00	0.00	0.00
			Total	100.00	77.00	12.00	11.00
5	7.31	181.10 a	With Damage	7.50	6.50	1.00	0.00
			Malformed	7.50	6.00	0.50	1.00
			Full	79.50	64.50	5.00	10.00
			Translucent	5.50	5.00	0.50	0.00
			Empty	0.00	0.00	0.00	0.00
			Total	100.00	82.00	7.00	11.00
6	15.41	152.75 b	With Damage	14.50	0.00	0.00	14.50
			Malformed	9.00	0.00	0.00	9.00
			Full	74.50	0.00	0.00	74.50
			Translucent	2.00	0.00	0.00	2.00
			Empty	0.00	0.00	0.00	0.00
			Total	100.00	0.00	0.00	100.00
7	12.43	150.12 b	With Damage	13.50	0.00	1.00	12.50
			Malformed	8.50	0.00	0.00	8.50
			Full	66.00	5.00	16.00	45.00
			Translucent	11.00	0.50	0.00	10.50
			Empty	1.00	0.00	0.00	1.00
			Total	100	5.5	17.5	77.5
9	12.13	122.37 b	With Damage	2.86	0.57	0.00	2.29
			Malformed	13.71	0.50	0.00	18.86
			Full	73.15	50.86	16.57	5.14
			Translucent	9.71	1.14	2.29	6.28
			Empty	0.57	0.00	0.00	0.57
			Total	100	53.0	18.85	27.48
10	12.97	130.75 b	With Damage	4.00	0.00	1.00	3.00
			Malformed	4.00	1.00	1.00	2.00
			Full	89.00	35.50	41.50	12.00
			Translucent	3.00	1.50	1.50	0.00
			Empty	0.00	0.00	0.00	0.00
CV%	24.29	25.84	Total	100.00	38.00	44.00	17.00

Variable	CF 1	CF 2	CF3	Commonalities
Damaged seeds	-0.946731	-0.151523	0.256522	0.985062
Malformed seeds	-0.129165	0.735010	0.359755	0.686347
Full seed	-0.555155	0.812620	0.038336	0.970018
Translucent seed	0.061374	0.976348	0.068189	0.961672
Empty seed	-0.215213	0.893092	0.125038	0.859564
Viability	0.892711	-0.116919	-0.397600	0.968688
Vigor	0.804339	-0.160130	-0.526274	0.949568
Decayed seed	0.965279	-0.174975	-0.119778	0.976726
Pixel Density	-0.007635	0.261585	0.962605	0.995092
Water content	-0.645389	0.118204	0.718400	0.946599
Eigenvalue	5.63	2.21	1.44	-
Total change (%)	56.36	22.14	14.47	-
Accumulated variation (%)	56.36	78.51	92.99	-
Explained variance (%)	40.63	31.26	21.09

Variable	Wd	Mf	Full	T	Empty	F	Vigor	Det	Pixel D	WC
Wd	1.00	0.16^ns	-0.65^ns	0.07^ns	0.28^ns	-0.75^ns	-0.63^ns	0.90*	0.20^ns	0.47^ns
Mf	-	1.00	-0.77^ns	0.63^ns	0.53^ns	-0.25^ns	-0.32^ns	0.35^ns	-0.50^ns	0.49^ns
Full	-	-	1.00	-0.74^ns	-0.79^ns	0.58^ns	0.56^ns	-0.69^ns	0.24^ns	-0.51^ns
T	-	-	-	1.00	0.93*	-0.12^ns	-0.17^ns	0.10^ns	-0.33^ns	0.11^ns
Empty	-	-	-	-	1.00	-0.41^ns	-0.46^ns	0.36^ns	-0.38^ns	0.28^ns
F	-	-	-	-	-	1.00	0.98*	-0.92*	0.43^nd	-0.85*
Vigor	-	-	-	-	-	-	1.00	-0.87*	0.57^ns	-0.87*
Det	-	-	-	-	-	-	-	1.00	-0.16^ns	0.71^ns
Pixel D	-	-	-	-	-	-	-	-	1.00	-0.72^ns
WC	-	-	-	-	-	-	-	-	-	1.00

Brasil

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Seed Longevity of Enterolobium contortisiliquum (Vell.) Morong

Longevidade de sementes de Enterolobium contortisiliquum (Vell.) Morong

Abstract: