Viability and performance of wheat seedlings after artificial seed aging

Seed deterioration is a continuous irreversible process that affects cell structures and molecules and compromises the physiological quality of seeds. This study aimed to assess the effect of artificial wheat seed deterioration on germination and seedling performance. The TBIO Toruk wheat cultivar was used, with seeds submitted to different artificial aging times (2, 3, 4, 5, 6 and 7 days). The original and aged seed lots were submitted to laboratory germination and seedling performance tests (seedling length, seedling dry weight and endosperm dry weight). Alpha-amylase activity, electrical conductivity and the oxidative stress marker malondialdehyde were quantified. The exudate resulting from the electrical conductivity test was separated to quantify total soluble sugars, soluble proteins and phosphorus. The deterioration process increased lipid peroxidation and decreased initial alpha-amylase activity. During germination, the most deteriorated lots exhibited greater solute loss and lower alpha-amylase synthesis capacity. The lots with the greatest deterioration showed reduced viability and produced worse-performing seedlings.

Index terms:
alpha-amylase; deterioration; malondialdehyde; physiological quality; Triticum aestivum L

Authorship

Matheus Santin Padilha

Universidade do Estado de Santa Catarina, Centro de Ciências Agroveterinárias, UDESC/CAV, 88520-000, Lages, SC, Brasil.Universidade do Estado de Santa CatarinaBrazilLages, SC, BrazilUniversidade do Estado de Santa Catarina, Centro de Ciências Agroveterinárias, UDESC/CAV, 88520-000, Lages, SC, Brasil.

http://orcid.org/0000-0001-6622-9252

Cileide Maria Medeiros Coelho ^**Corresponding author E-mail: cileide.souza@udesc.br

http://orcid.org/0000-0001-9528-7371

Natalia Carolina Moraes Ehrhardt-Brocardo

Centro de Educação Santa Teresinha, CEST/Sagrada, 89520-000, Curitibanos, SC, Brasil.Centro de Educação Santa TeresinhaBrasilCuritibanos, SC, BrasilCentro de Educação Santa Teresinha, CEST/Sagrada, 89520-000, Curitibanos, SC, Brasil.

http://orcid.org/0000-0002-0397-448X

*Corresponding author
E-mail: cileide.souza@udesc.br

SCIMAGO INSTITUTIONS RANKINGS

Tables | Formulas

Tables (4)
Formulas (23)

Thumbnail

Table 1
Germination first count (FC), normal seedlings (NS), abnormal seedlings (AS) and dead seeds (DS) in seven wheat seed lots before and after 2, 3, 4, 5, 6 and 7 days of artificial aging.

Thumbnail

Table 2
Seedling length (SL), seedling dry weight (SDW), endosperm dry weight (EDW), and alpha-amylase activity at three and five days of germination in seven wheat seed lots before and after 2, 3, 4, 5, 6 and 7 days of artificial aging.

Thumbnail

Table 3
Malondialdehyde (MDA), alpha-amylase activity and electrical conductivity (EC) in seven wheat seed lots before and after 2, 3, 4, 5, 6 and 7 days of artificial aging.

Thumbnail

Table 4
Total soluble sugars (TSS), soluble protein (SP) and phosphorus (P) in the electrical conductivity exudate of seven wheat seed lots before and after 2, 3, 4, 5, 6 and 7 days of artificial aging.

Thumbnail

Table 1 Germination first count (FC), normal seedlings (NS), abnormal seedlings (AS) and dead seeds (DS) in seven wheat seed lots before and after 2, 3, 4, 5, 6 and 7 days of artificial aging.

Deterioration	FC	NS	AS	DS
(Days)	------------------------- % -----------------------
0	91 a	94 a	3 c	3 e
2	78 b	86 b	7 c	7 d
3	59 c	77 c	12 b	11 c
4	49 d	72 c	14 b	14 c
5	38 e	61 d	20 a	19 b
6	25 f	55 d	22 a	23 b
7	11 g	24 e	24 a	52 a
$y = a x + b$	$y = - 11.73 x + 95.25$	$y = 5.82 x - 4.09$	$y = - 9.01 x + 102.12$	$y = 3.19 x + 1.97$
R²	0.98	0.98	0.87	0.75
p-value	0.000	0.000	0.000	0.029
CV (%)	11.98	5.86	23.86	25.19

Means followed by the same letter in the column do not differ significantly according to the Scott- Knott test at 5% probability. n=28. R²: Coefficient of determination. CV: Coefficient of variation.

Table 2 Seedling length (SL), seedling dry weight (SDW), endosperm dry weight (EDW), and alpha-amylase activity at three and five days of germination in seven wheat seed lots before and after 2, 3, 4, 5, 6 and 7 days of artificial aging.

3 Days of Germination
Deterioration	SL	SDW	EDW	Alpha-amylase
(Days)	cm.seedling^-1	mg.seedling^-1	mg.seed^-1	U.mg^-1 protein
0	6.78 a	3.59 a	24.40 a	3.34 a
2	5.20 b	2.96 b	25.14 a	2.98 a
3	4.62 c	2.98 b	26.86 a	3.15 a
4	4.21 c	2.34 c	26.50 a	2.56 b
5	3.48 d	2.30 c	26.39 a	2.41 b
6	3.04 d	1.99 c	26.35 a	2.31 b
7	1.10 e	1.18 d	27.52 a	1.66 c
$y = a x + b$	$y = - 0.72 x + 6.86$	$y = - 0.31 x + 3.69$	$y = 0.37 x + 24.72$	$y = - 0.23 x + 3.50$
R²	0.95	0.93	0.73	0.88
p-value	0.000	0.000	0.004	0.000
CV (%)	12.42	16.49	5.23	12.19
5 Days of Germination
Deterioration (Days)	SL	SDW	EDW	Alpha-amylase
Deterioration (Days)	cm.seedling^-1	mg.seedling^-1	mg.seed^-1	U.mg^-1
0	17.04 a	7.92 a	15.80 b	9.32 a
2	14.92 b	7.67 a	16.03 b	7.84 b
3	14.42 b	8.01 a	16.78 b	6.55 c
4	12.22 c	7.44 a	17.32 b	6.08 c
5	9.94 d	5.60 b	17.42 b	6.67 c
6	7.44 e	4.31 c	21.07 a	6.01 c
7	5.02 f	3.22 d	22.97 a	5.41 c
$y = a x + b$	$y = - 1.75 x + 18.32$	$y = - 0.71 x + 9.06$	$y = 0.99 x + 14.37$	$y = - 0.51 x + 8.81$
R²	0.94	0.98	0.76	0.85
p-value	0.000	0.001	0.004	0.000
CV (%)	11.34	10.94	8.99	8.60

Means followed by the same letter in the column do not differ significantly according to the Scott- Knott test at 5% probability. n=28. R²: Coefficient of determination. CV: Coefficient of variation.

Table 3 Malondialdehyde (MDA), alpha-amylase activity and electrical conductivity (EC) in seven wheat seed lots before and after 2, 3, 4, 5, 6 and 7 days of artificial aging.

Deterioration (days)	MDA (nmol.mL^-1)	Alpha-amylase (U.mg^-1 protein)	EC (µS.cm^-1 .g^-1)
0	1.08 b	0.18 a	37.40 c
2	1.22 a	0.15 a	36.97 c
3	1.21 a	0.13 a	36.58 c
4	1.25 a	0.08 b	41.23 b
5	1.27 a	0.09 b	48.66 a
6	1.33 a	0.10 b	41.74 b
7	1.28 a	0.07 b	42.21 b
$y = a x + b$	$y = 0.0292 x + 1.124$	$y = - 0.0155 x + 0.175$	$y = 1.1291 x + 36.33$
R²	0.72	0.82	0.41
p-value	0.000	0.012	0.000
CV (%)	5.44	37.88	6.05

Means followed by the same letter in the column do not differ significantly according to the Scott- Knott test at 5% probability. n=28. R²: Coefficient of determination. CV: Coefficient of variation.

Table 4 Total soluble sugars (TSS), soluble protein (SP) and phosphorus (P) in the electrical conductivity exudate of seven wheat seed lots before and after 2, 3, 4, 5, 6 and 7 days of artificial aging.

Deterioration (days)	TSS (µg.mL^-1)	SP (µg.mL^-1)	P (µg.mL^-1)
0	13.04 b	120.10 b	0.77 b
2	14.72 b	139.63 b	0.76 b
3	17.67 b	135.91 b	0.76 b
4	24.02 a	168.43 a	0.87 a
5	30.58 a	166.97 a	0.90 a
6	29.55 a	148.60 b	0.89 a
7	31.83 a	169.66 a	0.96 a
$y = a x + b$	$y = 3.126 x + 11.00$	$y = 6.527 x + 124.73$	$y = 0.029 x + 0.73$
R²	0.90	0.67	0.79
p-value	0.000	0.001	0.002
CV (%)	19.87	10.40	11.19

Means followed by the same letter in the column do not differ significantly according to the Scott- Knott test at 5% probability. n=28. R²: Coefficient of determination. R²: Coefficient of determination. CV: Coefficient of variation.

y = a x + b

y = - 11.73 x + 95.25

y = 5.82 x - 4.09

y = - 9.01 x + 102.12

y = 3.19 x + 1.97

y = a x + b

y = - 0.72 x + 6.86

y = - 0.31 x + 3.69

y = 0.37 x + 24.72

y = - 0.23 x + 3.50

y = a x + b

y = - 1.75 x + 18.32

y = - 0.71 x + 9.06

y = 0.99 x + 14.37

y = - 0.51 x + 8.81

y = a x + b

y = 0.0292 x + 1.124

y = - 0.0155 x + 0.175

y = 1.1291 x + 36.33

y = a x + b

y = 3.126 x + 11.00

y = 6.527 x + 124.73

y = 0.029 x + 0.73

How to cite

copy

ABRATES - Associação Brasileira de Tecnologia de Sementes Av. Juscelino Kubitschek, 1400 - 3° Andar, sala 31 - Centro,, CEP 86020-000 Londrina/PR - Londrina - PR - Brazil
E-mail: jss@abrates.org.br

Acompanhe os números deste periódico no seu leitor de RSS

Versão para download de PDF

PDF

Inglês

Versões e tradução automática

Versão original do texto

English

Tradução automática

Google Translator
Microsoft Translator

Como citar

RIS

BIBTEX

Outros formatos de citação e exportação:

SciELO - Scientific Electronic Library Online
Rua Dr. Diogo de Faria, 1087 – 9º andar – Vila Clementino 04037-003 São Paulo/SP - Brasil
E-mail: scielo@scielo.org

Leia a Declaração de Acesso Aberto

Métricas

Viability and performance of wheat seedlings after artificial seed aging

PlumX

Mensagem

[1] *Corresponding author
E-mail: cileide.souza@udesc.br