A redução da umidade e da temperatura permite prolongar o armazenamento de sementes, exceto para aquelas sensíveis à dessecação (recalcitrantes), o que dificulta a inclusão destas em bancos de conservação ex situ. Uma forma de controlar a atividade metabólica e a entrada ou saída de água na semente é o uso de substâncias químicas osmoticamente ativas, como o polietilenoglicol (PEG). Neste estudo avaliou-se o efeito do armazenamento de sementes recalcitrantes de Eugenia brasiliensis com controle hídrico em meio osmótico. Os resultados demonstraram que a manutenção dessas sementes em potencial hídrico entre -1 e -2 MPa permitiu ampliar a capacidade de conservação da viabilidade em armazenamento. Demonstraram, também, que a ampliação dessa conservação, diferentemente do que ocorre com a redução da temperatura, não necessariamente está associada à redução das taxas metabólicas.

Termos de indexação:
deterioração; PEG; recalcitrância; espécies tropicais

Autoria

Aline Testoni Cécel

Instituto de Pesquisas Ambientais, Av. Miguel Stéfano 3687, São Paulo, SP 04301-902, Brasil. Instituto de Pesquisas AmbientaisBrasilSão Paulo, SP, BrasilInstituto de Pesquisas Ambientais, Av. Miguel Stéfano 3687, São Paulo, SP 04301-902, Brasil.

https://orcid.org/0000-0003-3467-6968

Claudio José Barbedo ^**Corresponding author E-mail: cjbarbedo@yahoo.com.br

https://orcid.org/0000-0002-4417-7495

*Corresponding author
E-mail: cjbarbedo@yahoo.com.br

SCIMAGO INSTITUTIONS RANKINGS

Tabelas

Tabelas (7)

Thumbnail

Table 1
O₂ consumption, CO₂ release and respiratory quotient (RQ) of Eugenia brasiliensis seeds, incubated at four temperatures. Means followed by the same letter in the column do not differ from each other (Tukey, 5%).

Thumbnail

Table 2
O₂ consumption, CO₂ release and respiratory quotient (RQ) of Eugenia brasiliensis seeds, incubated at 8 °C in airtight glass jars, loose or in paper rolls with water or PEG solution at -2 and -8 MPa. Means followed by the same letter in the column do not differ from each other (Tukey, 5%).

Thumbnail

Table 3
Germination and formation of normal seedlings (Seedlings) of Eugenia brasiliensis seeds stored for 60 and 120 days at two temperatures (25 and 8 °C) and in perforated plastic bags (Plastic) or airtight glass jars (Glass). Means followed by the same letter (lowercase comparing packages, uppercase comparing temperatures), within each storage period, do not differ from each other (Tukey, 5%).

Thumbnail

Table 4
Water content (%) of Eugenia brasiliensis seeds without storage (0 days) or stored at 8 °C for 60, 180, 360 and 540 days in plastic bags, loose (Control) or in paper rolls with PEG solution of different water potentials (-1 to -9 MPa). Means followed by the same letter in the columns do not differ from each other (Tukey, 5%).

Thumbnail

Table 5
Water potential (MPa) of Eugenia brasiliensis seeds without storage (0 days) or stored at 8 °C for 60, 180, 360 and 540 days in plastic bags, loose (Control) or in paper rolls with PEG solution of different water potentials (-1 to -9 MPa). Means followed by the same letter in the columns do not differ from each other (Tukey, 5%).

Thumbnail

Table 6
Germination (%) of Eugenia brasiliensis seeds without storage (0 days) or stored at 8 °C for 60, 180, 360 and 540 days in plastic bags, loose (Control) or in paper rolls with PEG solution of different water potentials (-1 to -9 MPa). Means followed by the same letter in the columns do not differ from each other (Tukey, 5%).

Thumbnail

Table 7
Production of normal seedlings (%) of Eugenia brasiliensis seeds without storage (0 days) or stored at 8 °C for 60, 180, 360 and 540 days in plastic bags, loose (Control) or in paper rolls with PEG solution of different water potentials (-1 to -9 MPa). Means followed by the same letter in the columns do not differ from each other (Tukey, 5%).

Table 1 O₂ consumption, CO₂ release and respiratory quotient (RQ) of Eugenia brasiliensis seeds, incubated at four temperatures. Means followed by the same letter in the column do not differ from each other (Tukey, 5%).

Temperature (°C)	O₂ consumption (μmol.gDM^-1.h^-1)	CO₂ release (μmol.gDM^-1.h^-1)	RQ
35	7.07 a	3.84 a	0.55 b
25	3.60 b	2.12 b	0.59 b
15	0.71 c	0.40 c	0.61 b
8	0.29 d	0.23 d	0.83 a

Table 2 O₂ consumption, CO₂ release and respiratory quotient (RQ) of Eugenia brasiliensis seeds, incubated at 8 °C in airtight glass jars, loose or in paper rolls with water or PEG solution at -2 and -8 MPa. Means followed by the same letter in the column do not differ from each other (Tukey, 5%).

Incubation	O₂ consumption (μmol.gDM^-1.h^-1)	CO₂ release (μmol.gDM^-1.h^-1)	RQ
Loose	0.25 a	0.16 a	0.71 b
Water	0.23 a	0.14 a	0.63 b
PEG -2 MPa	0.29 a	0.17 a	0.64 b
PEG -8 MPa	0.16 b	0.15 a	0.98 a

Table 3 Germination and formation of normal seedlings (Seedlings) of Eugenia brasiliensis seeds stored for 60 and 120 days at two temperatures (25 and 8 °C) and in perforated plastic bags (Plastic) or airtight glass jars (Glass). Means followed by the same letter (lowercase comparing packages, uppercase comparing temperatures), within each storage period, do not differ from each other (Tukey, 5%).

Storage (days)	Temperature (°C)	Package	Germination (%)	Seedlings (%)
60	25	Plastic	100 a A	93 a A
		Glass	0 b B	0 b B

	8	Plastic	90 a A	87 a A
		Glass	87 a A	77 a A

120	25	Plastic	100 a A	70 a B
		Glass	0 b B	0 b B

	8	Plastic	93 a A	93 a A
		Glass	87 a A	77 b A

Table 4 Water content (%) of Eugenia brasiliensis seeds without storage (0 days) or stored at 8 °C for 60, 180, 360 and 540 days in plastic bags, loose (Control) or in paper rolls with PEG solution of different water potentials (-1 to -9 MPa). Means followed by the same letter in the columns do not differ from each other (Tukey, 5%).

Water potential of the solution	Storage periods (days)
Water potential of the solution	Initial	60		180		360		540
Control	50.8	53.2	a	57.3	a	62.9	a	63.8	a
-1 MPa		50.4	ab	52.1	b	52.4	b	53.0	b
-2 MPa		49.6	b	50.1	b	51.1	b	51.1	b
-3 MPa		48.0	bc	45.7	c	47.4	c	47.1	c
-7 MPa		45.3	c	42.6	d	38.0	e	41.3	d
-8 MPa		45.0	c	40.9	d	42.0	d	42.4	d
-9 MPa		45.2	c	41.6	d	38.5	e	42.0	d

Table 5 Water potential (MPa) of Eugenia brasiliensis seeds without storage (0 days) or stored at 8 °C for 60, 180, 360 and 540 days in plastic bags, loose (Control) or in paper rolls with PEG solution of different water potentials (-1 to -9 MPa). Means followed by the same letter in the columns do not differ from each other (Tukey, 5%).

Water potential of the solution	Storage periods (days)
Water potential of the solution	Initial	60		180		360		540
Control	-0.81	-0.90	c	-1.05	f	-1.01	e	-0.80	d
-1 MPa		-1.32	bc	-0.89	f	-1.74	d	-1.55	c
-2 MPa		-0.96	c	-2.19	e	-2.46	c	-2.16	bc
-3 MPa		-1.74	ab	-3.21	d	-2.97	c	-2.70	b
-7 MPa		-2.25	a	-4.84	c	-4.12	b	-4.07	a
-8 MPa		-1.76	ab	-5.10	b	-4.23	ab	-4.04	a
-9 MPa		-2.15	a	-6.30	a	-4.89	a	-4.36	a

Table 6 Germination (%) of Eugenia brasiliensis seeds without storage (0 days) or stored at 8 °C for 60, 180, 360 and 540 days in plastic bags, loose (Control) or in paper rolls with PEG solution of different water potentials (-1 to -9 MPa). Means followed by the same letter in the columns do not differ from each other (Tukey, 5%).

Water potential of the solution	Storage periods (days)
Water potential of the solution	Initial	60		180		360		540
Control	100	100	a	30	c	0	c	0	c
-1 MPa		90	a	97	a	90	a	87	a
-2 MPa		97	a	100	a	90	a	80	a
-3 MPa		100	a	80	b	63	b	23	b
-7 MPa		40	b	0	d	0	c	0	c
-8 MPa		53	b	3	d	0	c	0	c
-9 MPa		13	c	0	d	0	c	0	c

Table 7 Production of normal seedlings (%) of Eugenia brasiliensis seeds without storage (0 days) or stored at 8 °C for 60, 180, 360 and 540 days in plastic bags, loose (Control) or in paper rolls with PEG solution of different water potentials (-1 to -9 MPa). Means followed by the same letter in the columns do not differ from each other (Tukey, 5%).

Water potential of the solution	Storage periods (days)
Water potential of the solution	Initial	60		180		360		540
Control	77	77	a	23	b	0	b	0	a
-1 MPa		70	a	70	a	53	a	13	a
-2 MPa		70	a	73	a	50	a	10	a
-3 MPa		77	a	60	a	30	a	7	a
-7 MPa		17	b	0	b	0	b	0	a
-8 MPa		30	b	0	b	0	b	0	a
-9 MPa		7	b	0	b	0	b	0	a

Como citar

copiar

ABRATES - Associação Brasileira de Tecnologia de Sementes Av. Juscelino Kubitschek, 1400 - 3° Andar, sala 31 - Centro,, CEP 86020-000 Londrina/PR - Londrina - PR - Brazil
E-mail: jss@abrates.org.br

Acompanhe os números deste periódico no seu leitor de RSS

Versão para download de PDF

PDF

Inglês

Versões e tradução automática

Versão original do texto

English

Tradução automática

Google Translator
Microsoft Translator

Como citar

RIS

BIBTEX

Outros formatos de citação e exportação:

SciELO - Scientific Electronic Library Online
Rua Dr. Diogo de Faria, 1087 – 9º andar – Vila Clementino 04037-003 São Paulo/SP - Brasil
E-mail: scielo@scielo.org

Leia a Declaração de Acesso Aberto

Métricas

Mensagem

[1] *Corresponding author
E-mail: cjbarbedo@yahoo.com.br