Three new neo-clerodanes, antiscorbuticane A, antiscorbuticane B and antiscorbuticane C, and known compounds glutinol, friedelin, 5,7-dihydroxyflavanone (pinocembrin), 5-hydroxy-3,6,7,4'-tetramethoxyflavone, 5-hydroxy-3,6,7,3',4'-pentamethoxyflavone (artemetin), 5,4'-dihydroxy-3,6,7,3'-tetramethoxyflavone (penduletin) and 5,3',4'-trihydroxy-3,6,7-trimethoxyflavone (chrysosplenol D), were isolated from the methanol extract of Tinnea antiscorbutica. Antiscorbuticane B exhibited no mutagenic activity at doses of up to 250 µg per plate (Ames test) and did not induce micronucleus formation in the V79 cell line at doses of up to 100 µg mL-1.
Tinnea antiscorbutica; neo-clerodanes; mutagenic activity; cytotoxic activity; genotoxicity
