Anatomia foliar e parâmetros fotossintéticos de Vellozia squamata Pohl (Velloziaceae) desenvolvendo-se sob diferentes intensidades luminosas ao longo do cultivo in vitro

Calazans Júnior, Elson Rangel; Silveira, Conceição Eneida dos Santos; Freitas-Neto, Olegário Garcia de; Melo, Dario Martins Palhares de; Pereira, Luiz Alfredo Rodrigues; Gomes, Sueli Maria

Acessibilidade / Reportar erro

Brasil
SciELO.org - Rede SciELO
Blog SciELO em Perspectiva

Abrir menu

Brasil

Español English

Hoehnea

Submissão de manuscritos
Sobre o periódico
Corpo Editorial
Instruções aos autores
Contato

sumário « anterior atual seguinte »

Resumo (Inglês)
Resumo (Português)

Texto (Inglês)

Download PDF (Inglês)

Compartilhe

Compartilhe
E-mail
Facebook
Twitter
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley

Sumário

Compartilhe
E-mail
Facebook
Twitter
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley

Resumo (Inglês)
Resumo (Português)

Texto (Inglês)

Download PDF (Inglês)

Article • Hoehnea 49 • 2022 • https://doi.org/10.1590/2236-8906-109/2020 copiar

Anatomia foliar e parâmetros fotossintéticos de Vellozia squamata Pohl (Velloziaceae) desenvolvendo-se sob diferentes intensidades luminosas ao longo do cultivo in vitro

Autoria SCIMAGO INSTITUTIONS RANKINGS

RESUMO

O presente trabalho avalia parâmetros fotossintéticos e a anatomia foliar de Vellozia squamata Pohl (Velloziaceae) em cada etapa da produção in vitro, sendo as plantas silvestres o grupo-controle. A abordagem anatômica foi feita para a porção mediana foliar. As curvas fotossintéticas foram obtidas com analisador de gases por infravermelho. As plântulas in vitro apresentaram folhas mais delgadas e menos desenvolvidas, as silvestres apresentaram folhas mais espessas e desenvolvidas e aquelas em aclimatação exibiram características intermediárias. As plântulas in vitro não foram capazes de absorção líquida de carbono. As plântulas jovens de casa-de-vegetação exibiram taxa líquida de assimilação de carbono inferior às selvagens. Em conclusão, para o cultivo in vitro as intensidades luminosas para a produção de mudas devem ser melhor ajustadas.

Palavras-chave:
aclimatação; Cerrado; conservação; cultivo; sombreamento

Autoria

Elson Rangel Calazans Júnior

Universidade de Brasília, Instituto de Ciências Biológicas, Campus Universitário Darcy Ribeiro, Bloco E s/n 1º andar, Asa Norte, 70919-900 Brasília, DF, BrazilUniversidade de BrasíliaBrazilBrasília, DF, Brazil Universidade de Brasília, Instituto de Ciências Biológicas, Campus Universitário Darcy Ribeiro, Bloco E s/n 1º andar, Asa Norte, 70919-900 Brasília, DF, Brazil

http://orcid.org/0000-0003-1744-7477

Conceição Eneida dos Santos Silveira

http://orcid.org/0000-0002-2854-2835

Olegário Garcia de Freitas-Neto

http://orcid.org/0000-0002-4009-9602

Dario Martins Palhares de Melo ³3. Corresponding author: dariompm@unb.br

http://orcid.org/0000-0001-7409-1521

Luiz Alfredo Rodrigues Pereira

http://orcid.org/0000-0001-8204-2586

Sueli Maria Gomes

http://orcid.org/0000-0002-6234-7777

3. Corresponding author: dariompm@unb.br

Author Contributions

Elson Calazans: Contribution to data collection; Contribution to data analysis and interpretation; Contribution to critical revision; Contribution to manuscript preparation. Conceição Eneida: Contribution in the concept and design of the study; Contribution to manuscript preparation; Contribution to critical revision, adding intellectual content. Olegário Garcia: Contribution to data collection. Dario Palhares: Contribution in the concept and design of the study; Contribution to data analysis and interpretation; Contribution to manuscript preparation; Contribution to critical revision, adding intellectual content. Luiz Alfredo: Contribution in the concept and design of the study; Contribution to manuscript preparation; Contribution to critical revision, adding intellectual content. Sueli Gomes: Contribution in the concept and design of the study; Contribution to manuscript preparation; Contribution to critical revision, adding intellectual content.

Conflicts of interest

There is no conflict of interest.

SCIMAGO INSTITUTIONS RANKINGS

Figuras | Tabelas

Figuras (6)
Tabelas (1)

Thumbnail

Figure 1.
Light-response curve of A (net carbon assimilation rate) of leaves (n=10) of Vellozia squamata Pohl (Velloziaceae) in field conditions during the rainy season under different values of photosynthetically active radiation (PAR). Non-linear adjustment by the equation A_meas: 8.57 (1-e^{-0.001658(PAR-80)})

Thumbnail

Figure 2.
Light-response curve of A (net carbon assimilation rate) of leaves (n=10) of seedlings of Vellozia squamata Pohl (Velloziaceae) acclimatizing in greenhouse conditions under different values of photosynthetically active radiation (PAR). Non-linear adjustment by the equation A_meas: 3.42(1-e^{-0.0176833(PAR-60)})

Thumbnail

Figure 3.
Light-response curve of A (net carbon assimilation rate) of leaves (n=10) of plantlets of Vellozia squamata Pohl (Velloziaceae) in vitro conditions under different values of photosynthetically active radiation (PAR). No adjustment of the curve was carried out.

Thumbnail

Figure 4.
Light-response curve of A (net CO₂ assimilation rate) of leaves (n=10) of Vellozia squamata Pohl (Velloziaceae) seedlings obtained by in vitro propagation and growing in a garden during the rainy season under different values of photosynthetically active radiation (PAR). Non-linear adjustment by the equation A_meas: 4.97 (1-e^{-0.00125(PAR-60)})

Thumbnail

Figure 5.
Plants of Vellozia squamata Pohl (Velloziaceae). a-b. In the field, showy lilac flower (b). c. In vitro condition. d. Greenhouse condition. e. Plants produced in vitro now growing at the garden. Bars: A = 1 m, B = 5 cm, C = 1 cm, D = 5 cm, E = 1 cm.

Thumbnail

Figure 6.
Leaf anatomy of Vellozia squamata Pohl (Velloziaceae). a-d. Plants from the field (b-c: details of a): adult (a-c) and young (d) leaves, showing the early formation of the stomatal crypts and later differentiation of the cuticle, subepidermal fibres, palisade parenchyma, and the vascular bundle sheath. e. Plants from micropropagation, not acclimatized: absence of stomatal crypts and subepidermal fibres. f. Plants from acclimatization similar to the adult leaf of the field, but with less deep crypts. g. Plants growing at garden condition. Arrows: stomata. AP: aqueous parenchyma, Ct: cuticle, Fi: fibres, PP: palisade parenchyma (elongated cells), SC: stomatal crypt, SP: spongy parenchyma (isodiametric cells). Bars a, d-g = 50 µm, b-c = 25 µm.

Thumbnail

Table 1.
Parameters of the experimental treatments with Vellozia squamata Pohl (Velloziaceae). A max: maximal CO₂ absorption rate, µmol CO₂.m^-2.s^-1 ; E: transpiration rate, mmol H₂O .m^-2.s^-1 ; LCP: light compensation point, µmol photon.m^-2.s^-1 ; LSP: light saturation point, µmol photon.m^-2.s^-1 ; L max: maximal PAR to which the plants are exposed, µmol photon.m^-2.s^-1 ; Chlorophyll: chlorophyll content, unities of chlorophyll (CCI); WUE: water use efficiency (µmol CO₂/mmol H₂O). Letters compare data in the same column. Distinct letters mean statistically significant difference (P<0.05) by Student´s T-test.

Figure 1. Light-response curve of A (net carbon assimilation rate) of leaves (n=10) of Vellozia squamata Pohl (Velloziaceae) in field conditions during the rainy season under different values of photosynthetically active radiation (PAR). Non-linear adjustment by the equation A_meas: 8.57 (1-e^{-0.001658(PAR-80)})

Figure 2. Light-response curve of A (net carbon assimilation rate) of leaves (n=10) of seedlings of Vellozia squamata Pohl (Velloziaceae) acclimatizing in greenhouse conditions under different values of photosynthetically active radiation (PAR). Non-linear adjustment by the equation A_meas: 3.42(1-e^{-0.0176833(PAR-60)})

Figure 3. Light-response curve of A (net carbon assimilation rate) of leaves (n=10) of plantlets of Vellozia squamata Pohl (Velloziaceae) in vitro conditions under different values of photosynthetically active radiation (PAR). No adjustment of the curve was carried out.

Figure 4. Light-response curve of A (net CO₂ assimilation rate) of leaves (n=10) of Vellozia squamata Pohl (Velloziaceae) seedlings obtained by in vitro propagation and growing in a garden during the rainy season under different values of photosynthetically active radiation (PAR). Non-linear adjustment by the equation A_meas: 4.97 (1-e^{-0.00125(PAR-60)})

Figure 5. Plants of Vellozia squamata Pohl (Velloziaceae). a-b. In the field, showy lilac flower (b). c. In vitro condition. d. Greenhouse condition. e. Plants produced in vitro now growing at the garden. Bars: A = 1 m, B = 5 cm, C = 1 cm, D = 5 cm, E = 1 cm.

Figure 6. Leaf anatomy of Vellozia squamata Pohl (Velloziaceae). a-d. Plants from the field (b-c: details of a): adult (a-c) and young (d) leaves, showing the early formation of the stomatal crypts and later differentiation of the cuticle, subepidermal fibres, palisade parenchyma, and the vascular bundle sheath. e. Plants from micropropagation, not acclimatized: absence of stomatal crypts and subepidermal fibres. f. Plants from acclimatization similar to the adult leaf of the field, but with less deep crypts. g. Plants growing at garden condition. Arrows: stomata. AP: aqueous parenchyma, Ct: cuticle, Fi: fibres, PP: palisade parenchyma (elongated cells), SC: stomatal crypt, SP: spongy parenchyma (isodiametric cells). Bars a, d-g = 50 µm, b-c = 25 µm.

Table 1. Parameters of the experimental treatments with Vellozia squamata Pohl (Velloziaceae). A max: maximal CO₂ absorption rate, µmol CO₂.m^-2.s^-1 ; E: transpiration rate, mmol H₂O .m^-2.s^-1 ; LCP: light compensation point, µmol photon.m^-2.s^-1 ; LSP: light saturation point, µmol photon.m^-2.s^-1 ; L max: maximal PAR to which the plants are exposed, µmol photon.m^-2.s^-1 ; Chlorophyll: chlorophyll content, unities of chlorophyll (CCI); WUE: water use efficiency (µmol CO₂/mmol H₂O). Letters compare data in the same column. Distinct letters mean statistically significant difference (P<0.05) by Student´s T-test.

Treatments	A max	E	LCP	LSP	Lmax	Chlorophyll	WUE
Cerrado	8.57±1.07 a	4.76± 0.90 a	80	900	1,925	24.6±3.97 a	1.8
Greenhouse	3.42±0.28 b	0.92±0.54 b	60	200	412	2.99±1.48 b	3.7
In vitro	n/a	3.44±0.03 c	n/a	n/a	40	1.37±0.44 c	n/a
Garden	4.97 ± 1.43 d	1.16 ± 0.06 b	60	900	1,925	8.70 ± 3.2 d	4.3

Como citar

copiar

Instituto de Pesquisas Ambientais Av. Miguel Stefano, 3687 , 04301-902 São Paulo SP / Brasil, Tel.: 55 11 5067-6057, Fax; 55 11 5073-3678 - São Paulo - SP - Brazil
E-mail: hoehneaibt@gmail.com

Acompanhe os números deste periódico no seu leitor de RSS

Versão para download de PDF

PDF

Inglês

Versões e tradução automática

Versão original do texto

English

Tradução automática

Google Translator
Microsoft Translator

Como citar

RIS

BIBTEX

Outros formatos de citação e exportação:

SciELO - Scientific Electronic Library Online
Rua Dr. Diogo de Faria, 1087 – 9º andar – Vila Clementino 04037-003 São Paulo/SP - Brasil
E-mail: scielo@scielo.org

Leia a Declaração de Acesso Aberto

Métricas

Anatomia foliar e parâmetros fotossintéticos de Vellozia squamata Pohl (Velloziaceae) desenvolvendo-se sob diferentes intensidades luminosas ao longo do cultivo in vitro

PlumX

Mensagem

[1] 3. Corresponding author: dariompm@unb.br