Ultrasonic therapy modulates the expression of genes related to neovascularization and inflammation in fibroblasts

Terapia ultrassônica modula a expressão de genes relacionados à neovascularização e inflamação em fibroblastos

Abstract

Introduction:

In the rehabilitation of musculoskeletal injuries, ultrasound is widely used in clinical practice.

Objective:

To evaluate the effects of pulsed ultrasonic therapy on the viability and modulation of genes involved in inflammation (IL-6) and neovascularization (VEGF) processes of L929 fibroblast cells.

Methods:

For irradiation with ultrasound the cells were subdivided into groups: G1 (without irradiation), G2 (0.3 W/cm²-20%) and G3 (0.6 W/cm²-20%), with periods of treatment at 24, 48 and 72 hours. The cell viability assay was analyzed by the MTT method and gene modulation was analyzed by RT-qPCR method.

Results:

After the comparative analysis between groups, only G2 and G3 (48-hour) presented statistically significant differences in relation to the control. In relation to the gene expression, the selection of the groups analyzed was delimited according to the comparative analysis of the values obtained by the MTT test. After the achievement of RT-qPCR, it could be observed that in G2 the amount of VEGF gene transcripts increased by 1.125-fold compared to endogenous controls, and increased 1.388-fold in G3. The IL-6 gene, on the other hand, had its transcripts reduced in both G2 (5.64x10^-9) and G3 (1.91x10^-6).

Conclusion:

Pulsed ultrasound in L929 fibroblasts showed a significant biostimulatory effect in the 48-hour period, with increased cell viability, and the same effect in the modulation of gene expression related the neovascularization and inflammation, mediating the acceleration of the tissue repair cascade.

Keywords:
Rehabilitation; Fibroblasts; Gene expression; Regeneration; Ultrasonic therapy