An isolate of Sugarcane mosaic virus (SCMV) associated with mosaic symptoms in the RB925268 sugarcane clone was identified in Paraná state. Axilary buds of 4 to 8 mm used as explants were regenerated into plantlets using MS medium with and without ribavirin antiviral, at concentrations of 10 to 60 mg/L. Ribavirin was toxic to sugarcane at all concentrations, resulting in plant death at concentrations of 30 mg/L or higher. The complete elimination of SCMV was obtained in MS medium with 25 mg/L ribavirin concentration, confirmed by sap inoculation into Sorghum bicolor cv. Rio and by RT-PCR.
micropropagation; virazole
