In bean (Phaseolus vulgaris) producing areas of Paraná State, Bean rugose mosaic virus (BRMV) has been found in mixed infections with Bean golden mosaic virus (BGMV) increasing the severity of symptoms and crop losses. The association of Cowpea severe mosaic virus (CPSMV) with bud blight symptoms in soybean (Glycine max) plants was recently observed in Paraná for the first time. In this work RT-PCR products of 600 bp of BRMV-PR RNA2 and 594 bp of CPSMV-PR RNA2 were cloned and sequenced. The amino acid sequences coded by the RNA2 segment from these isolates were compared with sequences of the same RNA2 genomic region of six comovirus species from the GenBank. The CPSMV-PR showed 85% homology with the CPSMV RNA2 from the GenBank. Moreover, the BRMV-PR isolate showed 39 and 44% homology with CPSMV and Bean pod mottle virus (BPMV), respectively. This work shows, for the first time, partial sequencing data for BRMV. This data may be helpful for the complete molecular characterization of this virus and for establishing strategies to obtain virus resistant plants.
