Lipidic compounds from the muscle of white shrimp (<i>Litopenaeus vannamei</i>): chemical structure and effect on the proliferation and morphology of human cancer cell lines

DE LA REÉ-RODRÍGUEZ, Sandra Carolina; LÓPEZ-SAIZ, Carmen María; EZQUERRA-BRAUER, Josafat Marina; SANTACRUZ-ORTEGA, Hisila del Carmen; PLASCENCIA-JATOMEA, Maribel; HERNÁNDEZ-ZAZUETA, Martin Samuel; SANDOVAL-PETRIS, Edgar; MARTINEZ-CRUZ, Oliviert; YEPIZ-PLASCENCIA, Gloria; MEDINA-MENDEZ, Isabel

doi:10.1590/fst.86822

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Original Article • Food Sci. Technol 42 • 2022 • https://doi.org/10.1590/fst.86822 copy

Lipidic compounds from the muscle of white shrimp (Litopenaeus vannamei): chemical structure and effect on the proliferation and morphology of human cancer cell lines

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

Cancer represents the second leading cause of death worldwide, therefore, the search for chemoprotective agents is on the rise. The muscle of white shrimp (Litopenaeus vannamei) is one of the species analyzed as it has been reported as a source of compounds with antiproliferative activity. The aim of this study was to evaluate the effect of shrimp muscle-isolated compounds on cell proliferation and morphology of human cancer cell lines. The muscle underwent a process of extraction and fractionation of compounds; their effect on cell viability assays (MTT) on lung adenocarcinoma (A549), prostate carcinoma (22 Rv-1), invasive breast adenocarcinoma (MDA MB 231), colon carcinoma (HTC 116), cervix adenocarcinoma (HeLa) and non-cancerous retinal cells (ARPE-19) was measured. Morphological changes were observed using fluorescence microscopy and chemical structure data was obtained using nuclear magnetic resonance. Fraction named C5 showed the highest antiproliferative activity on HCT-116 and MDA-MB-231, without significantly affecting the control cells. Subfractions C5-3 and C5-4 presented significant antiproliferative potential in MDA-MB-231; this cell line showed morphological changes that could be related to apoptosis, and spectroscopic analysis revealed the presence of b-carotene and eicosapentaenoic acid in C5, nevertheless, further studies are needed to determine the effect of each compound.

Keywords:
bioactive compounds; chemoprevention; cancer

Fraction	Concentration (μg/mL)	Cell line (%)
	Concentration (μg/mL)	HeLa	22-Rv1	A549	HCT-116	MDA-MB-231	ARPE-19
C1	25	89.2 ± 21.0	79.8 ± 7.1^ab	104.9 ± 5.3^a	114.4 ± 14.9^ab	97.3 ± 10.9^ab	100.1 ± 0.3^ab
	50	108.5 ± 13.3	72.9 ± 18.2^abc	80.9 ± 5.7^cd	110.0 ± 11.6^ab	101.2 ± 13.1^ab	104.0 ± 3.5^ab
	100	118.5 ± 15.9	79.4 ± 11.5^abc	77.6 ± 5.4^cd	92.7 ± 3.9^ab	104.7 ± 13.5^a	91.1 ± 8.9^b
	200	103.7 ± 1.8	65.1 ± 7.1^abc	78.4 ± 4.8^cd	97.0 ± 4.02^ab	102.8 ± 15.4^a	100.2 ± 4.5^ab
C2	25	97.1 ± 20.9	69.8 ± 0.6^abc	84.4 ± 3.5^abcd	108.7 ± 0.6^ab	98.1 ± 8.2^ab	105.8 ± 9.1^ab
	50	128.5 ± 38.2	79.7 ± 5.8^ab	83.6 ± 4.9^bcd	119.4 ± 13.8^ab	100.8 ± 10.1^ab	101.2 ± 16.2^ab
	100	118.8 ± 14.9	74.7 ± 7.3^abc	83.8 ± 4.5^abcd	106.8 ± 27.8^ab	96.9 ± 17.7^ab	83.2 ± 6.4^b
	200	108.5 ± 4.6	67.7 ± 4.1^abc	82.5 ± 3.1^bcd	103.3 ± 4.9^ab	91.6 1 ± 3.5^ab	102.7 ± 7.2^ab
C3	25	88.6 ± 21.9	68.04 ± 13.0^abc	103.5 ± 8.3^ab	83.1 ± 7.5^ab	104.1 ± 9.04^a	101.7 ± 8.8^ab
	50	105.1 ± 12.8	59.9 ± 7.3^abc	89.2 ± 7.3^abcd	79.9 ± 10.7^ab	118.9 ± 9.4^a	108.4 ± 14.2^ab
	100	113.1 ± 5.9	53.8 ± 1.0^abc	86.1 ± 7.7^abcd	85.3 ± 7.8^ab	117.4 ± 18.1^a	82.1 ± 7.7^b
	200	124.6 ± 4.6	41.1 ± 3.3^c	89.2 ± 5.4^abcd	82.8 ± 8.6^ab	112.6 ± 20.1^a	109.7 ± 11.5^ab
C4	25	89.5 ± 11.9	65.8 ± 1.6^abc	86.9 ± 2.6^abcd	90.2 ± 4.8^ab	97.1 ± 4.2^ab	99.5 ± 6.2^ab
	50	114.2 ± 25.4	73.8 ± 0.1^abc	90.9 ± 2.6^abcd	104.3 ± 15.3^ab	105.1 ± 0.3^a	117.0 ± 10.6^ab
	100	99.4 ± 8.6	75.2 ± 0.5^abc	92.6 ± 1.5^abcd	95.9 ± 5.7^ab	99.0 ± 16.0^ab	100.4 ± 3.1^ab
	200	110.6 ± 14.0	75.4 ± 0.4^abc	96.8 ± 1.0^abc	107.3 ± 6.2^ab	105.8 ± 9.4^a	139.4 ± 9.3^a
C5	25	83.5 ± 19.9	77.4 ± 3.0^abc	98.0 ± 6.3^abc	100.9 ± 7.7^ab	98.8 ± 15.6^ab	113.0 ± 5.1^ab
	50	94.2 ± 19.4	80.3 ± 4.0^ab	90.3 ± 9.6^abcd	106.55 ± 4.5^ab	95.0 ± 11.3^ab	111.9 ± 8.6^ab
	100	121.4 ± 6.9	74.3 ± 0.6^abc	84.9 ± 4.6^abcd	96.5 ± 8.9^ab	76.0 ± 27.0^ab	92.6 ± 6.8^ab
	200	110.9 ± 10.4	46.7 ± 11.4^bc	41.5 ± 6.5^e	39.9 ± 11.1^b	37.3 ± 1.1^ab	80.1 ± 19.5^b
C6	25	87.7 ± 8.6	69.3 ± 0.3^abc	83.8 ± 1.1^abcd	101.7 ± 3.9^ab	98.0 ± 3.2^ab	105.5 ± 0.5^ab
	50	107.9 ± 13.8	79.3 ± 0.01^abc	85.3 ± 2.7^abcd	107.4 ± 6.8^ab	98.3 ± 7.1^ab	102.4 ± 13.4^ab
	100	100.1 ± 13.9	75.4 ± 1.6^abc	88.9 ± 4.9^abcd	95.2 ± 2.2^ab	90.6 ± 13.9^ab	99.0 ± 4.5^ab
	200	76.2 ± 10.4	69.6 ± 1.9^abc	83.9 ± 5^abcd	96.8 ± 5.6^ab	82.3 ± 12.3^ab	93.3 ± 11.7^ab
C7	25	95.5 ± 2.7	75.7 ± 8.2^abc	87.9 ± 3.4^abcd	94.5 ± 19.3^ab	101.5 ± 9.6^ab	118.0 ± 15.6^ab
	50	118.7 ± 8.9	90.3 ± 0.1^a	80.2 ± 8.5^cd	83.5 ± 15.2^ab	101.2 ± 8.0^ab	100.6 ± 7.3^ab
	100	107.6 ± 16.6	82.4 ± 2.4^ab	77.3 ± 8.1^cd	130.9 ± 38.6^a	97.2 ± 7.9^ab	105.0 ± 2.7^ab
	200	99.1 ± 12.0	71.9 ± 12.2^abc	74.6 ± 2.2^d	101.8 ± 22.5^ab	82.89 ± 2.4^ab	108.0 ± 7.5^ab

Sub-fraction	Concentration (μg/mL)	Cell lines (%)
Sub-fraction	Concentration (μg/mL)	HCT-116	MDA-MB-231	ARPE-19
C5-1	12.5	122.2 ± 7.3	82.6 ± 8.7^bcde	112.7 ± 5.4^ab
	25	116.9 ± 3.8	132.1 ± 9.8^ab	112.0 ± 17.7^ab
	50	118.4 ± 9.9	126.4 ± 9.2^abc	91.5 ± 9.9^abc
	100	97.9 ± 10.1	145.7 ± 13.8ª	82.0 ± 2.6^abcd
C5-2	12.5	81.2 ± 4.5	76.8 ± 6.2^cde	108.0 ± 9.8^ab
	25	91.3 ± 7.5	102.4 ± 8.1^abcde	131.0 ± 13.7ª
	50	101.7 ± 10.3	106.9 ± 11.8^abcde	101.6 ± 14.3^abc
	100	103.3 ± 8.45	95.1 ± 17.0^bcde	98.5 ± 7.6^abc
C5-3	12.5	97.5 ± 5.4	79.3 ± 10.1^cde	105.0 ± 14.5^ab
	25	82.8 ± 7.0	158.0 ± 10.0^a	124.2 ± 10.2^a
	50	94.3 ± 7.6	117.6 ± 12.4^abcd	101.1 ± 4.0^abc
	100	86.6 ± 8.3	14.1 ± 4.4^g	31.7 ± 10.5^d
C5-4	12.5	83.1 ± 7.0	134.9 ± 14.0^ab	118.3 ± 5.4^ab
	25	96.0 ± 6.1	92.1 ± 4.8^bcde	112.6 ± 16.9^ab
	50	96.4 ± 8.23	68.5 ± 3.7^defg	88.2 ± 12.7^abcd
	100	94.5 ± 7.4	18.4 ± 4.9^fg	44.3 ± 18.2^cd
C5-5	12.5	81.5 ± 7.3	102.9 ± 13.9^abcde	118.1 ± 9.5^ab
	25	111.7 ± 6.9	115.2 ± 9.7^abcd	100.6 ± 6.5^abc
	50	94.4 ± 14.0	109.7 ± 7.3^abcde	92.2 ± 12.4^abc
	100	87.7 ± 6.6	71.1 ± 11.8^cdef	61.6 ± 10.1^bcd
C5-6	12.5	89.6 ± 8.3	105.5 ± 9.7^abcde	77.3 ± 4.4^abcd
	25	101.0 ± 6.8	122.2 ± 13.1^abc	102.3 ± 7.7^abc
	50	96.6 ± 9.9	69.6 ± 7.2^defg	96.5 ± 12.0^abc
	100	87.7 ± 8.3	92.8 ± 10.8^bcde	91.1 ± 7.8^abcd
Cis-platin	100	11.3 ± 2.78	23.1 ± 3.5	37.5 ± 5.8
Astaxanthin	100	-	113.4 ± 14.7	-

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[1] *Corresponding author: carmen.lopezsaiz@unison.mx