Tripterygium glycoside ameliorates kidney injury in diabetic rats by regulating the PI3K/Akt signaling pathway

HOU, Dandan; SHANG, Sainan; LV, Juan; WANG, Shuling

doi:10.1590/fst.124721

Dandan HOU

Shuangqiao Hospital, Chaoyang, Beijing, China

Sainan SHANG

Shuangqiao Hospital, Chaoyang, Beijing, China

Juan LV

Shuangqiao Hospital, Chaoyang, Beijing, China

Shuling WANG ^**Corresponding author: wangshuling1223@163.com

Shuangqiao Hospital, Chaoyang, Beijing, China

http://orcid.org/0000-0003-0487-0030

*Corresponding author: wangshuling1223@163.com

Figures (5)
Tables (2)

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Figure 1
Observe the pathomorphological changes of renal tissue by HE staining (200×). Normal: The normal control group; Model: Diabetic induced kidney injury model group; Low: Model rats treated with 1mg/kg TG; Middle: The Model rats treated with 2 mg/kg TG; High: The Model rats treated with 8 mg/kg TG.

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Figure 2
The ultrastructure of rat kidney was observed by transmission electron microscope (10000×). Normal: The normal control group; Model: Diabetic induced kidney injury model group; Low: Model rats treated with 1mg/kg TG; Middle: The Model rats treated with 2 mg/kg TG; High: The Model rats treated with 8 mg/kg TG

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Figure 3
PTEN, PI3K, p-Akt protein expression by WB assay. Normal: The normal control group; Model: Diabetic induced kidney injury model group; Low: Model rats treated with 1mg/kg TG; Middle: The Model rats treated with 2 mg/kg TG; High: The Model rats treated with 8 mg/kg TG. ***: P < 0.001, vs. Normal; #: P < 0.05, ##: P < 0.01, ###: P < 0.001, vs. Model; $: P < 0.05, $$: P < 0.01, vs. Middle; &: P < 0.05, vs. Middle.

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Figure 4
Observed apoptosis cell by TUNEL in kidney tissues (200×). Normal: The normal control group; Model: Diabetic induced kidney injury model group; Low: Model rats treated with 1mg/kg TG; Middle: The Model rats treated with 2 mg/kg TG; High: The Model rats treated with 8 mg/kg TG. ***: P < 0.001, vs. Normal; #: P < 0.05, ##: P < 0.01, ###: P < 0.001, vs. Model; $: P < 0.05, $$: P < 0.01, vs. Middle; &: P < 0.05, vs. Middle.

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Figure 5
Bax, Caspase-3 and Bcl-2 protein expression in kidney tissues. Normal: The normal control group; Model: Diabetic induced kidney injury model group; Low: Model rats treated with 1mg/kg TG; Middle: The Model rats treated with 2 mg/kg TG; High: The Model rats treated with 8 mg/kg TG. ***: P < 0.001, vs. Normal; #: P < 0.05, ##: P < 0.01, ###: P < 0.001, vs. Model; $: P < 0.05, $$: P < 0.01, vs. Middle; &: P < 0.05, vs. Middle.

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Table 1
FBG, TG, TC and HDL-C levels in serum of difference groups (Mean±SD, n=9, mmol/L).

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Table 2
Urinary metabolic index level, renal weight, body weight and renal weight index in each group (Mean ± SD, n = 9).

Group	Dose	FBG	TG	TC	HDL-C
Normal		5.12 ± 0.50	0.33 ± 0.04	55.82 ± 1.88	1.05 ± 0.07
Model		23.34 ± 1.70***	0.57 ± 0.03***	94.99 ± 1.43***	0.69 ± 0.05***
Low	1 mg/kg	19.98 ± 2.81#	0.56 ± 0.03^# # P < 0.05,	84.87 ± 175#	0.81 ± 0.05#
Middle	2 mg/kg	13.70 ± 2.61##,$	0.48 ± 0.03##,$	72.48 ± 1.76##,$	0.83 ± 0.04##,$
High	8 mg/kg	9.23 ± 1.80^### ### P < 0.001, compared with Model; ,$$,&	0.41 ± 0.02###,$$,&	68.91 ± 1.21###,$$,&	0.91 ± 0.04###,$$,&

Group	Dose	BUN (mmol/L)	Scr (μmol/L)	24 h Urine protein (mg)	Kidney wight (mg)	Body weight (kg)	KI (mg/kg)
Normal		5.75 ± 0.03	31.49 ± 1.79	10.59 ± 1.46	1.46 ± 0.22	0.36 ± 0.02	4.08 ± 0.61
Model		18.87 ± 2.29^* ***** P < 0.001, compared with Normal;	52.71 ± 1.53***	65.59 ± 4.06***	2.45 ± 0.25***	0.28 ± 0.02***	8.89 ± 0.94***
Low	1 mg/kg	16.52 ± 1.68^# # P < 0.05;	40.37 ± 2.09#	52.62 ± 3.28#	2.11 ± 0.20#	0.27 ± 0.01#	7.77 ± 0.90#
Middle	2 mg/kg	14.00 ± 2.11^## ## P < 0.01; ,$	36.87 ± 1.90##,$	42.41 ± 3.98##,$	1.95 ± 0.14##,$	0.32 ± 0.03##,$	6.03 ± 0.67##,$
High	8 mg/kg	13.29 ± 2.45###,$$,&	34.71 ± 1.61###,$$,&	40.36 ± 1.76###,$$,&	1.71 ± 0.15###,$$,&	0.34 ± 0.02###,$$,&	5.15 ± 0.67###,$$,&

[1] *Corresponding author: wangshuling1223@163.com

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Tripterygium glycoside ameliorates kidney injury in diabetic rats by regulating the PI3K/Akt signaling pathway

Abstract