Antioxidant, antimicrobial and antiproliferative activities of fungal metabolite produced by Aspergillus flavus on in vitro study

Abstract

Regarding to the defiance came upon scientists to output natural products with potent biological activity from cheap sources and in an attempt to recover the average of antiproliferative agents, the employment of fungal metabolite in creating a cordial proper process was a prerequisite. In the present results, fungal metabolites were detected by thin layer chromatography. One fungal isolate out of ten isolates bioassayed, was showed to have the most potent antioxidant, antiproliferative and antimicrobial activity; this fungal isolate was identified as belonging to Aspergillus flavus (A. flavus). Fungal extract of A. flavus showed a high free radical scavenging activity using Diphenyl-1-picrylhydrazyl (DPPH) scavenging % with low a concentration of IC₅₀ and potent flavonoid content. The result of instrumental analysis using GC.Mass illustrates the presence of different potent products of A. flavus extract. In addition, A. flavus extract showed a promising antiproliferative activity. Inhibitory activity against Hepatocellular carcinoma cells was detected under these experimental conditions. This strategy can be further used to elicit new age drugs.

Keywords:
Aspergillus flavus; bioactive compounds; GC; DPPH; antibacterial; antifungal