Hard beans, due to inappropriate storage conditions, have their quality and acceptability affected. In order to use them as edible products it is necessary to conduct studies on technological processes to make possible their utilization. Therefore, the objective of this work was to characterize protein concentrates obtained from new and aged dry black common beans (Phaseolus vulgaris L.), Iapar 44 cultivar, as well as to evaluate their functional properties and their usage as food ingredient. The aging of beans was accelerated by storage at 41 °C and 75% RH for 30 and 60 days. The new sample, stored at 5 °C, was taken as the control. Protein concentrates were obtained by alkaline extraction with NaOH 0,02% followed by isoelectric precipitation (pH 4,5) with concentrated HCl. The traits evaluated were: yield, chemical composition, eletrophoretic pattern of proteins, and the functional properties of solubility, water absorption, gel formation, oil absorption, emulsifying capacity, emulsifying activity, emulsion stability, foam formation, and foam stability. The protein concentrate average yield was 15% and the concentrates presented 75% of protein with eletrophoretic pattern of proteins and solubility in water that varied with the storage time. The protein solubility of the concentrate was affected by pH, being lower at pHs 4 and 5. The functional properties of water absorption and gel formation increased with storage. The emulsifying activity and emulsion stability were affected by storage for 60 days. The other properties were not affected by storage. The results showed the possibility of using the protein concentrate from stored dry black beans as food ingredient.
acceptance; food ingredient; functionality; hard-bean; Phaseolus vulgaris; proteins
