SUFU reduced pancreatic cancer cell growth by Wnt/β-catenin signaling pathway

The aim of the present study was to investigate the function and mechanism of SUFU in treatment of pancreatic cancer. Patient with pancreatic cancer (n = 50) and normal volunteers (n = 30) were obtained from The Second Affiliated Hospital of Soochow University between May 2014 and April 2013. SUFU mRNA and protein expressions in patients with pancreatic cancer were reduced as compared to normal saline. OS and DFS of high expression of SUFU in pancreatic cancer were higher than those of low expression of SUFU in pancreatic cancer. The inhibition of SUFU promoted pancreatic cancer cell growth and migration; SUFU reduced pancreatic cancer cell growth and migration. SUFU suppressed Wnt/β-catenin signaling pathway in pancreatic cancer cell lines. SUFU reduced pancreatic cancer cell growth by Wnt/β-catenin signaling pathway, providing a novel possibility for an understanding of pancreatic cancer pathogenesis.

Keywords:
SUFU; β-catenin; pancreatic cancer

Authorship

Limin LIU

Department of Gastroenterology, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, ChinaThe Second Affiliated Hospital of Soochow UniversityChinaSuzhou, Jiangsu, ChinaDepartment of Gastroenterology, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China

Department of Gastroenterology, Yancheng First People’s Hospital, Yancheng, Jiangsu, ChinaYancheng First People’s HospitalChinaYancheng, Jiangsu, ChinaDepartment of Gastroenterology, Yancheng First People’s Hospital, Yancheng, Jiangsu, China

The Fourth Affiliated Hospital of Nantong University, Yancheng, Jiangsu, ChinaThe Fourth Affiliated Hospital of Nantong UniversityChinaYancheng, Jiangsu, ChinaThe Fourth Affiliated Hospital of Nantong University, Yancheng, Jiangsu, China

Duanmin HU ^**Corresponding author: duanminhu@21cn.com.

http://orcid.org/0000-0003-1360-4295

*Corresponding author: duanminhu@21cn.com.

Ethical approval This study was approved by the Ethics Committee of the The Second Affiliated Hospital of Soochow University and performed in accordance with the Declaration of Helsinki.

The author declare that they have no conflict of interest.

Limin Liu was the guarantor of integrity of the entire study, study concepts and design and analyzed the data. Duanmin Hu participated in literature research, experimental studies and helped to draft the manuscript. All authors read and approved the final manuscript.

SCIMAGO INSTITUTIONS RANKINGS

Figures

Figures (6)

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Figure 1
SUFU expression in patients with pancreatic cancer or cell lines. Heat map (A), SUFU mRNA expression (B) and protein expression (C, D) in patients with pancreatic cancer, SUFU mRNA expression in cell lines (E), OS and DFS of patients with pancreatic cancer (F, G). Normal, normal volunteers group; pancreatic cancer, pancreatic cancer group. ##p < 0.01 compared with normal volunteers group.

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Figure 2
SUFU reduced pancreatic cancer cell growth. SUFU mRNA expression (A), cell migration (B, C), cell growth (D), Edu positive cells (E, F), caspase-3/9 activity and LDH activity levels (G-I). Negative, negative mimic group; SUFU, over-expression of SUFU group. ##p < 0.01 compared with negative mimic group.

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Figure 3
The inhibition of SUFU promoted pancreatic cancer cell growth. SUFU mRNA expression (A), cell migration (B, C), cell growth (D), Edu positive cells (E, F), caspase-3/9 activity and LDH activity levels (G-I). Negative, negative mimic group; siSUFU, down-regulation of SUFU group. ##p < 0.01 compared with negative mimic group.

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Figure 4
SUFU suppressed Wnt/β-catenin signaling pathway in pancreatic cancer cell lines. Heat map (A), β-catenin expression (Immunofluorescence, B), SUFU and β-catenin protein expression (C-F) by over-expression of SUFU; SUFU and β-catenin protein expression (F-I) by down-regulation of SUFU. Negative, negative mimic group; SUFU, over-expression of SUFU group; siSUFU, down-regulation of SUFU group. ##p < 0.01 compared with negative mimic group.

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Figure 5
The activation of Wnt/β-catenin signaling pathway controlled the function of SUFU in pancreatic cancer cell lines. β-catenin protein expression (A, B, D), cell growth (C), cell migration (E, G), LDH activity levels (F), caspase-3/9 activity (I, K), Edu positive cells (J, H). Negative, negative mimic group; SUFU, over-expression of SUFU group; SUFU+ siβ-catenin, over-expression of SUFU and down-regulation of β-catenin group. ##p < 0.01 compared with negative mimic group; **p < 0.01 compared with over-expression of SUFU group.

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Figure 6
The inhibition of Wnt/β-catenin signaling pathway controlled the function of SUFU in pancreatic cancer cell lines. β-catenin protein expression (A, B, D), cell growth (C), cell migration (E, G), LDH activity levels (F), caspase-3/9 activity (I, K), Edu positive cells (J, H). Negative, negative mimic group; siSUFU, down-regulation of SUFU group; SUFU+ siβ-catenin, down-regulation of SUFU and over-expression of β-catenin group. ##p < 0.01 compared with negative mimic group; **p < 0.01 compared with down-regulation of SUFU group.

Figure 1 SUFU expression in patients with pancreatic cancer or cell lines. Heat map (A), SUFU mRNA expression (B) and protein expression (C, D) in patients with pancreatic cancer, SUFU mRNA expression in cell lines (E), OS and DFS of patients with pancreatic cancer (F, G). Normal, normal volunteers group; pancreatic cancer, pancreatic cancer group. ##p < 0.01 compared with normal volunteers group.

Figure 2 SUFU reduced pancreatic cancer cell growth. SUFU mRNA expression (A), cell migration (B, C), cell growth (D), Edu positive cells (E, F), caspase-3/9 activity and LDH activity levels (G-I). Negative, negative mimic group; SUFU, over-expression of SUFU group. ##p < 0.01 compared with negative mimic group.

Figure 3 The inhibition of SUFU promoted pancreatic cancer cell growth. SUFU mRNA expression (A), cell migration (B, C), cell growth (D), Edu positive cells (E, F), caspase-3/9 activity and LDH activity levels (G-I). Negative, negative mimic group; siSUFU, down-regulation of SUFU group. ##p < 0.01 compared with negative mimic group.

Figure 4 SUFU suppressed Wnt/β-catenin signaling pathway in pancreatic cancer cell lines. Heat map (A), β-catenin expression (Immunofluorescence, B), SUFU and β-catenin protein expression (C-F) by over-expression of SUFU; SUFU and β-catenin protein expression (F-I) by down-regulation of SUFU. Negative, negative mimic group; SUFU, over-expression of SUFU group; siSUFU, down-regulation of SUFU group. ##p < 0.01 compared with negative mimic group.

Figure 5 The activation of Wnt/β-catenin signaling pathway controlled the function of SUFU in pancreatic cancer cell lines. β-catenin protein expression (A, B, D), cell growth (C), cell migration (E, G), LDH activity levels (F), caspase-3/9 activity (I, K), Edu positive cells (J, H). Negative, negative mimic group; SUFU, over-expression of SUFU group; SUFU+ siβ-catenin, over-expression of SUFU and down-regulation of β-catenin group. ##p < 0.01 compared with negative mimic group; **p < 0.01 compared with over-expression of SUFU group.

Figure 6 The inhibition of Wnt/β-catenin signaling pathway controlled the function of SUFU in pancreatic cancer cell lines. β-catenin protein expression (A, B, D), cell growth (C), cell migration (E, G), LDH activity levels (F), caspase-3/9 activity (I, K), Edu positive cells (J, H). Negative, negative mimic group; siSUFU, down-regulation of SUFU group; SUFU+ siβ-catenin, down-regulation of SUFU and over-expression of β-catenin group. ##p < 0.01 compared with negative mimic group; **p < 0.01 compared with down-regulation of SUFU group.

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SUFU reduced pancreatic cancer cell growth by Wnt/β-catenin signaling pathway

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[1] *Corresponding author: duanminhu@21cn.com.