Figure 1
CLDN18 overexpression inhibits cell proliferation and induces cell apoptosis in gastric cancer cells. CLDN18 protein expression (A, B), cell proliferation (C), LDH activity (D), cell migration (E, F) and transferrer rate (G, H), cell apoptosis (I, J), caspase-3/9 activity (K, L). Negative, negative group; CLDN18, over-expression of CLDN18 group. ##P < 0.05 vs negative group.
Figure 2
CLDN18 downregulation enhances cell proliferation and reduced cell apoptosis in gastric cancer cells. CLDN18 protein expression (A, B), cell proliferation (C), LDH activity (D), cell migration (E, F) and transferrer rate (G, H), cell apoptosis (I, J), caspase-3/9 activity (K, L). Negative, negative group; siCLDN18, down-regulation of CLDN18 group. ##P < 0.05 vs negative group.
Figure 3
CLDN18 in gastric cancer patients and vivo model. Tumor volume and weight (A, B, C), Immunohistochemical for CLDN18 (D) in vivo model of gastric cancer, CLDN18 and CLDN18 protein expression (E, F, G), caspase-3/9 activity levels (H, I). Negative, negative group; siCLDN18, down-regulation of CLDN18 group. ##P < 0.05 vs negative group.
Figure 4
CLDN18 controls CLDN18 expression in gastric cancer cells. CLDN18 expression in patients with gastric cancer (A), overall survival (B), gene chip (C), network analysis graphics (D), IP for CLDN18 controls CLDN18 expression (E), CLDN18 protein expression (F, G) by over-expression of CLDN18; CLDN18 protein expression (H, I) by down-regulation of CLDN18. Negative, negative group; CLDN18, over-expression of CLDN18 group; siCLDN18, down-regulation of CLDN18 group. ##P < 0.05 vs negative group.
Figure 5
ARHGAP26 overexpression inhibits cell proliferation and induces cell apoptosis in gastric cancer cells. CLDN18 protein expression (A, B), cell proliferation (C), LDH activity (D), cell migration (E, F) and transferrer rate (G, H), cell apoptosis (I, J), caspase-3/9 activity (K, L). Negative, negative group; ARHGAP26, over-expression of ARHGAP26 group. ##P < 0.05 vs negative group.
Figure 6
ARHGAP26 downregulation enhances cell growth and reduced cell apoptosis of gastric cancer cells. CLDN18 protein expression (A, B), cell proliferation (C), LDH activity (D), cell migration (E, F) and transferrer rate (G, H), cell apoptosis (I, J), caspase-3/9 activity (K, L). Negative, negative group; siARHGAP26, down-regulation of ARHGAP26 group. ##P < 0.05 vs negative group.
Figure 7
Downregulation of ARHGAP26 rescues the effects of CLDN18-mediated tumor suppressive effects on gastric cancer cells. CLDN18 protein expression (A, B), cell proliferation (C), LDH activity (D), cell migration (E, F) and transferrer rate (G, H), cell apoptosis (I, J), caspase-3/9 activity (K, L). Negative, negative group; CLDN18, over-expression of CLDN18 group; CLDN18+siARHGAP26, over-expression of CLDN18 and down-regulation of ARHGAP26 group. ##P < 0.05 vs negative group; **P < 0.05 vs over-expression of CLDN18 group
Figure 8
ARHGAP26 in gastric cancer patients and vivo model. ARHGAP26 expression in patients with gastric cancer (A), overall survival (B), tumor volume and weight (C, D, E), Immunohistochemical for ARHGAP26 (F) in vivo model of gastric cancer, ARHGAP26 protein expression (G, H), caspase-3/9 activity levels (I, J). Negative, negative group; siARHGAP26, down-regulation of ARHGAP26 group. ##P < 0.05 vs negative group.
Figure 9
ARHGAP26 reduced the effects of CLDN18 downregulation in vivo model. Tumor volume and weight (A, B, C), ARHGAP26 protein expression (D, E), Immunohistochemical for ARHGAP26 (F), caspase-3/9 activity levels (G, H) in vivo model of gastric cancer. Negative, negative group; siCLDN18, down-regulation of CLDN18 group; siCLDN18+ARHGAP26, down-regulation of CLDN18 and over-expression of ARHGAP26 group. ##P < 0.05 vs negative group; **P < 0.05 vs down-regulation of CLDN18 group.
Figure 10
CLDN18-ARHGAP26 mediated tumor suppressive effects on gastric cancer cells by ABCG2 and ABCB1 pathway. Gene chip (A), network analysis graphics (B), ABCG2 and ABCB1 protein expression by over-expression of ARHGAP26 (C, D, E), ABCG2 and ABCB1 protein expression by down-regulation of ARHGAP26 (F, G, H), ABCG2 and ABCB1 protein expression by over-expression of CLDN18 (I, J, K), ABCG2 and ABCB1 protein expression by down-regulation of CLDN18 (L, M, N).
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