This study aimed to validate laboratory tested criteria to preserve vitamin C (Ascorbic Acid (AA) and Dehydroascorbic Acid (DHA)) in collard and tomato prepared in a Hospital Food Service (HFS). The criteria were: 24 hour-storage under refrigeration (10 °C), cleansing for 15 minutes, and distribution right after preparation. The consume exposure times, frequently used by the HFS, were also analyzed. High Performance Liquid Chromatography (HPLC) was the analysis method employed, using as mobile phase sodium phosphate 1 mM, EDTA 1 mM, all diluted in ultra pure water, and pH 3.0 adjusted with phosphoric acid. ANOVA (α = 0.05) was used to data analysis. No significant differences were found as to the content of vitamin C after the steps of vegetables handling showing high vitamin stability with the adopted criteria. However, a reduction of AA retention was observed in collard after 60 minutes of exposure to consumption (retention: 46.94%) and in tomato after 120 minutes of exposure (retention: 71.81%). The criteria contributed to control vitamin C loss in vegetables; therefore, their implementation is suggested to be applied in other HFS.
ascorbic acid; dehydroascorbic acid; tomato; collard; hospital food service
