Abstract
We aimed to optimize the culture condition for industrial effluent isolated Saccharomyces cerevisiae strains to reach the highest zinc biosorption, biomass, and protein production for human and animals. S. cerevisiae strains carrying ZRT and FET genes were isolated from effluent samples and identified using standard methods. Subsequently, the growth rate of yeasts in the presence of Zn2+, as well as the level of Zn2+ uptake by the yeast cells, were examined at 24-hour intervals. RT-PCR technique was applied to quantify the expression level of the target genes in yeast cells. The effect of the initial pH of culture medium was studied on the yeast growth rate, zinc absorption, and target genes expression. After setting the optimum pH, Kjeldahl method was applied for assessment of the total protein content of yeast cells. In the optimum conditions, S. cerevisiae showed the maximum growth rate, zinc uptake, and expression level of Zrt1 and Fet4. In addition, protein content of S. cerevisiae biomass in this optimum condition was above 50% (w/w). We demonstrated that S. cerevisiae species isolated from industrial effluents could be considered as highly promising candidates for producing Zn-enriched single cell protein. However, further research is believed to be required.
Keywords:
S. cerevisiae; Zinc; biosorption; single cell protein
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