Effect of mori folium and eucommiae cortex oral solution on immune function of chicken infected with IBDV

Abstract

Sangdu oral liquid (MFEC), a kind of traditional Chinese medicine, is used as a new immune enhancer for veterinary drugs or vaccines. In current study, we aimed to evaluate the effect and mechanism of MFEC on improving the immune response of B87 vaccine. Eighty SPF chickens were divided into blank control group (BC group), infectious bursal disease virus (IBDV) group (IBDV group), vaccine group (B87+IBDV group) and vaccine and Sangdu oral liquid combination group (MFEC+B87+IBDV group). In addition to the BC group, all other groups were infected with IBDV to establish immunosuppressive model. Chickens in both B87+IBDV and MFEC+B87+IBDV groups were immunized with B87 vaccine. Chickens in MFEC+B87+IBDV group were given MFEC for 7 days. Compared with B87+IBDV group, there were significantly lower morbidity and mortality, higher level of antibody, less tissue damage, lower expression of Bax and higher expression of Bcl-2 of MFEC+B87+IBDV group (P < 0.05). In MFEC+B87+IBDV group, the expression of IBDV-VP2, IL-6 and IFN-γ mRNA in bursal tissue was significantly lower than that in B87+IBDV group (P < 0.05). MFEC can effectively improve the immune response to B87 vaccine, and resist the invasion and replication of IBDV to avoid the tissue damage and apoptosis caused by IBDV infection.

Keywords:
Sangdu Oral Liquid; B87 vaccine; infectious bursal disease; antibody; apoptosis