Effects of high hydrostatic pressure on physicochemical and functional properties of soybean protein isolate

ZHAO, Fei; LIU, Xuemei; LIAN, Meng; YANG, Yongqi; LI, Chunlei; XU, Haicheng; CAO, Wenchao; ZHENG, Limin; DONG, Haizhou; WANG, Wentao

doi:10.1590/fst.95422

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Food Science and Technology

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Original Article • Food Sci. Technol 43 • 2023 • https://doi.org/10.1590/fst.95422 copy

Effects of high hydrostatic pressure on physicochemical and functional properties of soybean protein isolate

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

Impacts of high hydrostatic pressure (HHP) pretreatment on the physicochemical, functional and antioxidant properties of soybean protein isolate (SPI) were investigated. Changes in the secondary and tertiary structures were determined by Fourier transform infrared spectra and fluorescence spectra. The particle sizes reduced from 217.20 nm to 192.70 nm while the zeta potential increased. It’s revealed by scanning electron microscopy that the HHP treatment could result in the formation of large aggregation, leaving the SPI with irregular and uneven surface. Moreover, hollow spheres were also observed in SPI by means of dynamic and static light scattering. As a result, the foaming capacity of HHP-treated SPI and antioxidant activity of the HHP-treated SPI hydrolysates were improved significantly.

Keywords:
high hydrostatic pressure; soybean protein isolates; structure; ζ-potential; dynamic and static light scattering; functional properties

Entry	M_w (g/mol)	R_g (nm)	R_h (nm)	A₂	p (R_g/R_h)	Structure
Control	2.89 ± 0.17 × 10⁶	96 ± 5.4	122.97	-3.28 ± 0.65 × 10^-4	0.78	Hard sphere
100 MPa-5 min	2.57 ± 0.39 × 10⁸	100 ± 18	95.99	8 ± 3.8 × 10^-7	1.04	Hollow sphere
100 MPa-15 min	8.2 ± 0.33 × 10⁷	93 ± 4.8	89.86	4.31 ± 0.8 × 10^-6	1.03	Hollow sphere
100 MPa-30 min	3.11 ± 0.2 × 10⁸	96.1 ± 7.4	90.63	4.23 ± 0.38 × 10^-6	1.06	Hollow sphere
200 MPa-5 min	5.3 ± 1.3 × 10⁶	148 ± 20	188.69	-3.52 ± 0.83 × 10^-4	0.78	Hard sphere
200 MPa-15 min	9.3 ± 4.2 × 10⁶	152 ± 37	173.34	-1.8 ± 0.38 × 10^-4	0.93	Hollow sphere
200 MPa-30 min	6.8 ± 5.9 × 10⁷	351 ± 93	158.39	4 ± 11 × 10^-6	2.22	GCP
300 MPa-5 min	7.7 ± 5.2 × 10⁷	260 ± 60	176.29	6.7 ± 6.6 × 10^-6	1.47	GCM
300 MPa-15 min	2.9 ± 1.9 × 10⁶	169 ± 52	175.87	-1.13 ± 0.53 × 10^-6	0.96	Hollow sphere
300 MPa-30 min	9.5 ± 5.3 × 10⁶	161 ± 52	192.58	-4.5 ± 2.2 × 10^-4	0.84	Hard sphere
400 MPa-5 min	2.53 ± 0.9 × 10⁷	199 ± 29	201.73	-5 ± 4.2 × 10^-6	0.99	Hollow sphere
400 MPa-15 min	5.1 ± 2.8 × 10⁷	214 ± 46	210.93	-5 ± 17 × 10^-5	1.01	Hollow sphere
400 MPa-30 min	9.1 ± 7.1 × 10⁷	195 ± 64	138.21	-8 ± 570 × 10^-7	0.8	Hard sphere
500 MPa-5 min	1.3 ± 1.4 × 10⁸	259 ± 98	216.19	4.0 ± 4.6 × 10^-5	1.2	GCM
500 MPa-15 min	4.1 ± 2.8 × 10⁷	201 ± 57	190.8	-1.5 ± 1.8 × 10^-4	1.05	Hollow sphere
500 MPa-30 min	8.8 ± 6.7 × 10⁷	203 ± 64	197.59	2±20 × 10^-5	1.03	Hollow sphere

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[1] *Corresponding author: feizhaozhaofei@126.com; wangwt@sdau.edu.cn