The purpose of the present work was to evaluate different dosis effect of cyclophosphamide (CY) as an immunosuppressor in ovine. Thirty animals were grouped into four categories: groups I, II and III received 10, 25 e 40mg kg-1 of CY and group IV remained as control. All animals were immunized with brucelosis vaccine and six days after that, serum agglutination test was performed. During one week, blood lymphocyte count and gammaglobulin serum concentration were also daily evaluated. Deaths occured only in group III (40mgkg-1), so the drug seems to be safe in dosis under 25mg kg-1. Immunosuppression was found both in groups II (25mg/kg) and III (40mg kg-1), as it could be found in blood lymphocyte count and serum agglutination test. Authors concluded that 25mg kg-1 is the most adequate dosis to use CY for experimental immunosuppression models in ovine, considering the immunosuppression effect, safety and toxicity.
cyclophosphamide; immunosuppression; ovine
