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Sample size for estimating the average duration of larval, pupal and larval stage plus pupal periods in Microtheca spp. by bootstrap

The objective of this research was to determine the sample size (number of insects) to estimate the average duration for larval, pupal and larval + pupal periods for M. ochroloma and M. semilaevis. Adults of both species were collected at a 0.25ha experimental area of forage turnip (Raphanus sativus L.) and for laboratory rearing (temperature 25±2°C, relative humidity 60±10% and photoperíod 12 hours). Afterwards larval, pupal and larval + pupal periods were measured, in days, of 119 and 81 insects, respectively, M. ochroloma and M. semilaevis. It was calculated measures of central tendency and variability and determined the sample size using bootstrap with replacement of 10000 samples. For estimating the average larval, pupal and larval + pupal periods, with amplitude of bootstrap confidence interval of 95%, equal a day, 42 and 35 insects are sufficient, respectively for both M. ochroloma and M. semilaevis.

Raphanus sativus L.; beetles; resampling


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