Cotyledons were used as explants in order to test the effect of different vitamin mixtures and BAP concentrations on yellow passion fruit (Passiflora edulis f. flavicarpa Deg.) in vitro direct organogenesis. They basal medium was constituted of MS salts, supplemented with 1 and 2mg.<img SRC="http:/img/fbpe/cr/v32n2/a09img01.gif"> -1 of BAP (6-benzilaminopurina). Vitamin mixtures MS and B5 were tested. Every assay was done with 60 explants. The data were analyzed by Tukey's test. The medium supplemented with B5 vitaminic complex induced an increase of the capacity of cotyledons to regenerate in the presence of both BAP concentrations, as well for the number of explants with buds as for the number of buds per explant. The analysis of ploidy level of regenerated plants showed a number of chromosomes apparently normal and typical for this specie (2n=18).
organogenesis; tissue culture; cytology; Passiflora edulis f. flavicarpa
