Isozymic analyses were used to investigate biochemical variations of somaclones from strawberry cultivar Vila Nova, obtained starting from calli. Analyses were performed using young leaves collected from plants during the in vitro multiplication and afther aclimatation at greenhouse. The enzyme systems used for the analysis of the clones consisted of acid phosphatase and esterase (pH 6.5 and 8.3). During the in vitro culture, two patterns of acid phosphatase were detected among the regenerants, however for the esterase all the genotypes exhibited the same pattern of bands. At greenhouse, were found three patterns of bands different for acid phosphatase. Alterations were not verified in relation to the number and band position for esterase (pH 6.5), while, for esterase (pH 8.3) polymorphism was observed among the clones. Both enzyme systems were influenced by the growth condiction (in vitro and in vivo) and by the developing stage of the plants.
strawberry; in vitro culture; somaclonal variation; esterase; acid phosphatase
