<i>Brucella spp.</i> detection in blood, semen and vaginal swabs in dogs from the urban area of Cuiabá/MT, Brazil

Dias, Sara Maria Dantas da Nóbrega; Cruz, Thalita Priscila Peres Seabra da; Kuczmarski, Antônio Henrique; Nakazato, Luciano; Dutra, Valeria; Paz, Regina Célia Rodrigues da

doi:10.1590/0103-8478cr20220455

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MICROBIOLOGY • Cienc. Rural 53 (12) • 2023 • https://doi.org/10.1590/0103-8478cr20220455 copy

Brucella spp. detection in blood, semen and vaginal swabs in dogs from the urban area of Cuiabá/MT, Brazil

Detecção de Brucella spp. em sangue, sêmen e swab vaginal de cães da região metropolitana de Cuiabá/MT

Authorship SCIMAGO INSTITUTIONS RANKINGS

ABSTRACT:

Brucellosis is a chronic contagious infectious zoonosis that affects the reproductive system of animals, causing economic and health losses. This study diagnosed Brucella spp. in commercial kennels, comparing PCR positivity in different biological samples (blood, semen, and vaginal secretion), as well as correlating these findings with reproductive indices. Hence, we analyzed dogs from kennels in the neighboring cities of Cuiabá and Várzea Grande/MT, Brazil. The reproductive histories of the animals were obtained and blood samples were collected from all animals (n=35); in addition, semen samples were collected from males (n=9) and vaginal swabs were collected from females (n=24) to perform polymerase chain reactions (PCR) for brucellosis. The findings indicated that vaginal swab PCR is an effective test to identify Brucella spp. For males, there were more positive results when testing blood samples, possibly because the male animals were at the beginning stage of infection.

Key words:
PCR; infertility; reproduction; zoonosis

Universidade Federal de Santa Maria Universidade Federal de Santa Maria, Centro de Ciências Rurais , 97105-900 Santa Maria RS Brazil , Tel.: +55 55 3220-8698 , Fax: +55 55 3220-8695 - Santa Maria - RS - Brazil
E-mail: cienciarural@mail.ufsm.br

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[1] E-mail: sara.conpleta@hotmail.com. ^*Corresponding author

Animal	Volume (mL)	Motility (%)	Vigor (1-5)	pH	Membrane Integrity (%)	Acrosome Integrity (%)
1’’	3.3	50	3	7	58	47
4’’’	7.7	75	4	6	42	69
5’’	2.8	70	3	6	55	66
6’’	17.1	80	4	6	61	83
7	10.3	90	5	6	78	89
8	3.4	95	5	6	71	91
9	15.3	80	4	6	83	77
10’’	0.5	25	1	7	31	23
11’	2.5	80	3	6	52	85
Média/DP	6.86 ±6.04	72.78±22	3.67±1.22	6.22 ±0.44	59±16.67	70±22.36

Animal	Consistency(1-3)	Concentration(x10⁶/mL)	Primary Defects(%)	Secondary Defects(%)	Total Defects (%)
1”	3	20	4	20	24
2	3	-	-	-	-
4”’	3	13	2	35	37
5’’	3	38	1	31	32
6’’	2	20	2	25	27
7	3	42	6	16	22
8	3	54	2	13	15
9	2	48	5	14	19
10’’	3	19	4	29	33
11’	2	15	3	38	41
Média/DP	3±0.48	29.89 ±15.5	3.22±1.64	24.56±9.29	27.78±8.61

Blood	---------------Swab--------------		Total
	Positive	Negative
Positive	4	4	8
Negative	6	10	16
Total	10	14	24

------------Agreement Kappa-----------			---------McNemar----------
P-value	Value	Agreement	P-value	X ²
0.2928	0.1176	Weak	0.7518	0.1

Blood	---------------Semen-------------		Total
	Positive	Negative
Positive	1	4	5
Negative	1	3	4
Total	2	7	9

----------Agreement Kappa----------			---------McNemar-------
P-value	Value	Agreement	P-value	X ²
0.2928	0.1176	Weak	0.3711	0.8