<i>In vitro</i> conservation of ‘Florida Rough’ lemon plants

Carvalho, Mariane de Jesus da Silva de; Souza, Antônio da Silva; Santos, Emanuela Barbosa; Soares Filho, Walter dos Santos; Ledo, Carlos Alberto da Silva; Costa, Eva Maria Rodrigues; Souza, Fernanda Vidigal Duarte

doi:10.1590/0103-8478cr20210276

Acessibilidade / Reportar erro

Brasil

Español English

sumário « anterior atual seguinte »

Sumário

Biology • Cienc. Rural 52 (12) • 2022 • https://doi.org/10.1590/0103-8478cr20210276 copy

In vitro conservation of ‘Florida Rough’ lemon plants

Conservação in vitro de plantas de limoeiro ‘Rugoso da Florida’

Authorship SCIMAGO INSTITUTIONS RANKINGS

ABSTRACT:

The establishment of minimum growth conditions is essential for in vitro germplasm conservation. Changes to the basic medium and carbon source concentrations are important factors for reducing plant growth in vitro. This study adjusted a protocol for the in vitro conservation of ‘Florida Rough’ lemon plants. Microcuttings (approximately 1 cm) from plants that were previously cultivated in vitro were inoculated into test tubes with 10 mL of woody plant medium (WPM) at different concentrations (1/1, 1/2 and 1/4) and supplemented with 0, 12.5, 25 and 50 g.L^-1 of sucrose, solidified with 7 g.L^-1 agar and adjusted to pH 5.8. The experiment was completely randomized in a 3 x 4 factorial design with 15 replications and was maintained under controlled conditions for 360 days. After this period the plant height in cm (PH), the plant dry mass in g (PDM) and the 21 numbers of green leaves (NGL), senescent leaves (NSL) and microcuttings (NM) were evaluated. The variables that best explained the observed behavior of the ‘Florida Rough’ lemon plants were NGL and PH, with values of 61.63 and 35.08%, respectively. The original concentration of the WPM with the addition of 25 g. L^-1 of sucrose yielded the best ‘Florida Rough’ lemon plant growth reduction in vitro while maintaining the physiologically health of the plants.

Key words:
Citrus spp.; germplasm conservation; multivariate analysis; plant height; numbers of green leaves.

Universidade Federal de Santa Maria Universidade Federal de Santa Maria, Centro de Ciências Rurais , 97105-900 Santa Maria RS Brazil , Tel.: +55 55 3220-8698 , Fax: +55 55 3220-8695 - Santa Maria - RS - Brazil
E-mail: cienciarural@mail.ufsm.br

Acompanhe os números deste periódico no seu leitor de RSS

[1] E-mail: evamrc_9@hotmail.com.^*Corresponding author.

Variable	Sij	Sij (%)
PH	136.05	35.08
NGL	239.02	61.63
NSL	6.09	1.57
NM	6.44	1.66
PDM	0.22	0.06

Sources of variation	DF	--------------------------------------Mean Square---------------------------------------					(
		PH	NGL	NSL	NM	PDM
Sucrose	3	23.9901^**	1.1366^**	0.0588^ns	0.1167^*	0.0553^**	0.4256^**
WPM	2	20.4841^**	1.5227^**	0.0799^ns	0.0312^ns	0.0264^**	0.7781^**
Suc. x WPM	6	9.0674^**	0.1451^ns	0.1258^ns	0.0876^*	0.0098^**	0.6027^**
Error	164	1.4143	0.2539	0.1020	0.0325	0.0020
CV (%)		27.94	15.44	37.87	13.21	41.41
Overall average		4.2563	10.4318	0.3125	1.3977	0.1093

WPM Concentration	Equation	R² (%)	Optimal Dose	Estimated Value
---------------------------------------------------------------------Plant height--------------------------------------------------------------
1/1	${\hat{y}}^{**} = 3.1361 + 0.0715 x$	98.89	25.00	4.92
1/2	${\hat{y}}^{*} = 3.9946 + 0.0208 x$	54.14	25.00	4.51
1/4	${\hat{y}}^{*ns} = 3.60$	-	25.00	3.60¹
-----------------------------------------------------------Number of green leaves----------------------------------------------------------
1/1; 1/2; 1/4	${\hat{y}}^{**} = 11.3900 - 0.0429 x$	77.22	25.00	10.32
----------------------------------------------------------Number of microcuttings---------------------------------------------------------
1/1	${\hat{y}}^{*} = 0.9268 + 0.0387 x - 0.0005 x^{2}$	80.46	38.70	1.68
1/2	${\hat{y}}^{*ns} = 1.47$	-	0.00	1.47¹
1/4	${\hat{y}}^{*ns} = 1.37$	-	0.00	1.37¹
---------------------------------------------------------------Plant dry mass----------------------------------------------------------------
1/1	${\hat{y}}^{**} = 0.0560 + 0.0028 x$	87.48	50.00	0.20
1/2	${\hat{y}}^{**} = 0.0944 + 0.0013 x$	83.01	50.00	0.16
1/4	${\hat{y}}^{*ns} = 0.09$	-	0.00	0.09¹

	NGL	NSL	NM	PDM
PH	0.17^*	-0.15 ^ns	-0.13 ^ns	0.36^**
NGL		-0.17^*	0.39^**	0.39^**
NSL			0.14 ^ns	-0.18^*
NM				0.12 ^ns

WPM	Average
1/1	11.1897 a
1/2	10.7931 a
1/4	9.3500 b