The objective this study was to evaluate the effects of dietary protein levels in pirapitinga, Piaractus brachypomus, submited to extremely acidic or alkaline pH. Juveniles were fed for 20 days with three diets with different crude protein (CP) levels (25.3, 32.4 and 40.0%) and then separated in five groups (n=10, three replicates each) which were kept in 60 L aquaria and exposed to pH 3.0, 3.5, 7.0, 10, or 10.5. Fish were removed from aquaria when they showed loss of swimming balance, and then blood was collected and plasma separated for measurement of Na+, Cl- and K+ levels. The increase of dietary protein levels (up to 40.0% CP) provided some protection for pirapitinga at pH 3.5 or 10.0 because the time to lose equilibrium increased after acute exposure, but was not effective for compensating ion loss at very acidic (Na+ and Cl-) and alkaline (Cl-) pH.
pirapitinga; fish; ionoregulation; extreme pH
