The use of electrophoretic techniques to separate multiple molecular forms of enzymes has been used in the biological science, where differences in isozymes among tissues can be used efficiently on cultivar differentiation during any life cycle phase. In this paper, the variability of six drought resistant peanut lines was studied by isozymes analysis aiming to verify the possible relations between enzymatic descriptors and drought resistance character. Leaflets were analyzed by horizontal poliacrylamide gel electrophoresis technique and buffer continuos systems for the following systems: acid phosphatase (ACP), malate dehydrogenase (MDH), leucine aminopeptidase (LAP), peroxidase (POX) and esterase (EST). The phenotypic characterization of the genotypes allowed four group separations to ACP, three to LAP, two to MDH, and six to POX and EST. The IAC Tupã cultivar (drought sensitive) was differentiated to the others genotypes, specially as to Senegal 55 437 cultivar (drought resistant) by principal components analysis.
Arachis hypogaea; electrophoresis; enzyme; principals components
