Acessibilidade / Reportar erro

CANINE SEMEN CONSERVATION AT 37ºC IN COCONUT WATER BASED EXTENDERS

The aim of this study was to evaluate the canine semen diluted with coconut water extender, added of 3-indol acetic acid (IAA) and egg yolk at 37ºC. Sperm of six stud dogs was collected by digital manipulation and divided in three equal aliquots. Dilution was performed by the addiction of coconut water (extender 1), coconut water plus 4% of IAA (extender 2) and coconut water plus 4% of IAA and 20% of egg yolk (extender 3). Sperm motility, vigor and morphology immediately after dilution and periodically until 180 minutes, as well as media degradation motility rate (DMR), were evaluated. Extenders were compared by Whitney-Mann test (P<0.05). Statistical differences were not shown between the extenders up to 60 minutes. After 90 minutes, sperm motility on the extender A was superior to the other extenders (95%). The DMR on the extender A (7.5%) was significantly inferior to the other extenders. Thus, coconut water extender is adequate for the preservation of canine semen at 37ºC for 180 minutes.

canine semen; termorresistance; coconut water


Universidade Federal de Santa Maria Universidade Federal de Santa Maria, Centro de Ciências Rurais , 97105-900 Santa Maria RS Brazil , Tel.: +55 55 3220-8698 , Fax: +55 55 3220-8695 - Santa Maria - RS - Brazil
E-mail: cienciarural@mail.ufsm.br