Anticoagulants and their effects on the hematological and biochemical parameters of yellow-spotted amazon river turtle

Furtado, Yuri Ian Carvalho; Monteiro, Cristiellem Cardoso; Gonzales, Anai Paola Prissilla Flores; Costa, Maria de Nazaré Ferreira; Brasiliense, Alexandre Renato Pinto; Yoshioka, Eliane Tie Oba

doi:10.1590/0103-8478cr20210489

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BIOLOGY • Cienc. Rural 52 (12) • 2022 • https://doi.org/10.1590/0103-8478cr20210489 copy

Anticoagulants and their effects on the hematological and biochemical parameters of yellow-spotted amazon river turtle

Anticoagulantes e seus efeitos em parâmetros hematológicos e bioquímicos de tracajá

Authorship SCIMAGO INSTITUTIONS RANKINGS

ABSTRACT:

Knowledge of suitable methods and reagents for assessing the health condition of specimens of a given species is essential. The present study evaluated the efficacy of the heparin anticoagulants 5,000 I.U. mL^-1, Na₂EDTA, and K₃EDTA on the blood parameters of yellow-spotted amazon river turtle Podocnemis unifilis, employing different solutions for red blood cells count. The use of the various anticoagulants evaluated after 10 hours of storage efficiently inhibited coagulation in blood samples from P. unifilis. An increased number of erythrocytes was observed with the use of K₃EDTA 5% when compared with heparin. Statistically significant changes in the erythrocyte number were observed with the use of the different solutions. Solutions which featured sodium citrate and formaldehyde in their composition, allowed erythrocytes counting up to 120 hours after blood collection, without a change in values. The use of the heparin anticoagulants 5,000 I.U. mL^-1, Na₂EDTA 3%, Na₂EDTA 5%, K₃EDTA 3% was recommended in the hematological analysis of P. unifilis. Also recommended was the use of the formaldehyde-citrate solution containing 1.9 g of sodium citrate and 1.0 mL of formaldehyde (in 50 mL of distilled water) to perform red blood cells counts in yellow-spotted amazon river turtle.

Key words:
blood; Chelonia; erythrocytes; Podocnemis unifilis; tracajá

Universidade Federal de Santa Maria Universidade Federal de Santa Maria, Centro de Ciências Rurais , 97105-900 Santa Maria RS Brazil , Tel.: +55 55 3220-8698 , Fax: +55 55 3220-8695 - Santa Maria - RS - Brazil
E-mail: cienciarural@mail.ufsm.br

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[1] E-mail: eliane.yoshioka@embrapa.br. ^*Corresponding author

Parameters	Sodium heparin	Na₂EDTA 3%	Na₂EDTA 5%	K₃EDTA 3%	K₃EDTA 5%
Ht (%)	22.69±2.07^a	24.25±4.43ª	25.06±3.17^a	25.50±4.21^a	26.00±3.53^a
Hb (g dL^-1)	7.13±0.73^a	7.19±1.47ª	7.53±0.79^a	7.35±1.38^a	7.23±1.19ª
RBC (x 10⁶ μL^-1)	0.35±0.07^b	0.43±0.11^ab	0.38±0.04^ab	0.44±0.09^ab	0.49±0.09^a
MCV (fL)	632.87±84.70^a	424.21±111.15^b	631.84±78.20^a	633.60±106.55^a	495.45±114.07ª^b
MCHC (g dL^-1)	32.23±2.69^a	32.47±7.45^a	31.16±5.81^a	32.01±6.30^a	31.52±3.51ª
Glucose (mg dL^-1)	37.95±7.31ª	42.76±9.42^a	35.59±7.94^a	36.00±7.69^a	39.70±8.80^a
Protein (g dL^-1)	3.54±0.35ª	3.69±0.55^a	3.57±0.44^a	3.85±0.29^a	3.69±0.34^a
Albumin (g dL^-1)	1.31±0.26^a	1.33±0.20^a	1.48±0.13^a	1.46±0.12^a	1.49±0.19^a

	Solution A	Solution B	Solution C	Solution D	Solution E	Solution F
0h	100.00±48.99^aAB	112.00±64.19^aAB	62.00±16.43^aAB	130.00±23.45^aAB	138.00±73.96^aA	38.00±40.25^aB
24h	136.00±31.30^aA	128.00±58.05^aA	74.00±81.42^aA	120.00±43.01^aA	0.00±0.00^bB	0.00±0.00^bB
48h	158.00±57.62^aA	132.00±60.99^aAB	54.00±58.99^aBC	138.00±32.71^aAB	0.00±0.00^bC	0.00±0.00^bC
72h	82.00±45.50ª^bAB	92.00±32.71ª^AB	38.00±41.47^aBC	112.00±31.14ª^A	0.00±0.00^bC	0.00±0.00^bC
96h	16.00±35.78^bB	126.00±11.40^aA	0.00±0.00^aB	132.00±34.20^aA	0.00±0.00^bB	0.00±0.00^bB
120h	0.00±0.00^bB	140.00±14.14^aA	0.00±0.00^aB	120.00±42.43^aA	0.00±0.00^bB	0.00±0.00^bB