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ABSTRACT Objective: circCPA4 has been defined to be an oncogenic gene. This study examined whether circCPA4 regulates Prostate Cancer (PC) development and revealed its molecular mechanism. Methods: PC tissues and PC cell lines were collected, in which circCPA4/miR-491-5p/SHOC2 levels were evaluated by RT-qPCR and immunoblot. Colony formation assay and EdU assay assessed cell proliferation, flow cytometry measured apoptosis, and Transwell assessed invasion and migration. Ki-67, cleaved caspase-3, E-cadherin, and N-cadherin were evaluated by immunoblot. Based on the luciferase reporter assay and RIP assay the authors investigated the targeting relationship between circCPA4/miR-491-5p/SHOC2. The effect of circCPA4 on tumor growth was evaluated by xenotransplantation in nude mice. Results: circCPA4 and SHOC2 levels were abundant while miR-491-5p expression was low in PC. Loss of circCPA4 decreased the proliferation and EdU-positive rate of PC cells, enhanced apoptosis, and inhibited invasion, migration, and EMT. Upregulation of circCPA4 forced the malignant behaviors of PC cells, and this promotion could be abolished when miR-491-5p was overexpressed or SHOC2 was silenced. CircCAP4 competitively decoyed miR-491-5p mediating SHOC2 expression. circCAP4 suppression inhibited PC tumor growth. Conclusion: circCAP4 acts as a novel oncogenic factor in PC, accelerating the malignant behavior of PC cells via miR-491-5p/SHOC2 interaction. This novel ceRNA axis may be a potential target for PC drug development and targeted therapy in the future.

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Abstract Objectives: This experimental study focused on the intra- and inter-rater reproducibility of vertical bone level (VBL) measurements at strategic mini-implants (MI) using digital panoramic radiographs (PR). Study design: VBLs of 152 MIs for removable partial denture stabilization at 50 randomly chosen PRs from a clinical trial were digitally evaluated by three ratters. Rater deviations exceeding 0.5 mm were re-examined. The intra-class correlation coefficient (ICC) was applied to estimate reliability. The smallest detectable change (SDC) was interrelated to the minimal clinically important change of 0.2 mm. Results: The first measurement round revealed intra- and inter-rater ICCs of > 0.8. However, 28 sites (9 %) were unreadable, and 97 sites (32 %) revealed differences between observers of ≥ 0.5 mm. Following a consensus session and re-training, an additional 8 sites were excluded and all remaining VBL differences were ≤ 0.5 mm. Thus, the SDCs with 95 % credibility were improved from 0.73 to 0.31 mm in the intra-rater and from 1.52 to 0.34 mm in the interrater statistics. Given a 50 % credibility for this special setting, both the intra- and inter-rater SDCs were 0.11 mm. Conclusions: Digital PR can be reliably utilized to determine VBLs around MIs under conditions of at least two trained observers, mutual calibration sessions, and exclusion of unquantifiable radiographs. German Clinical Trials Register ID:DRKS00007589, www.germanctr.de

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Abstract Objective: This study aimed to develop and internally validate a prediction model for estimating the risk of spontaneous abortion in early pregnancy. Methods: This prospective cohort study included 9,895 pregnant women who received prenatal care at a maternal health facility in China from January 2021 to December 2022. Data on demographics, medical history, lifestyle factors, and mental health were collected. A multivariable logistic regression analysis was performed to develop the prediction model with spontaneous abortion as the outcome. The model was internally validated using bootstrapping techniques, and its discrimination and calibration were assessed. Results: The spontaneous abortion rate was 5.95% (589/9,895) 1. The final prediction model included nine variables: maternal age, history of embryonic arrest, thyroid dysfunction, polycystic ovary syndrome, assisted reproduction, exposure to pollution, recent home renovation, depression score, and stress score 1. The model showed good discrimination with a C-statistic of 0.88 (95% CI 0.87‒0.90) 1, and its calibration was adequate based on the Hosmer-Lemeshow test (p = 0.27). Conclusions: The prediction model demonstrated good performance in estimating spontaneous abortion risk in early pregnancy based on demographic, clinical, and psychosocial factors. Further external validation is recommended before clinical application.

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Highlights Effects of AGEs in Parkinson's disease patients. Recommendation healthy diet with a low AGE consumption. Parkinson's disease patients’ lower strength and worsened functional capacity.

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HIGHLIGHTS CS-31 seems to be appropriate for use in oncology and may help to monitoring adverse effects and HRQL.

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Highlights DM1 is associated with changes in skeletal muscle functionality. During pregnancy, the incidence of low back pain and pelvic pain is moderate and the presence of DM1 may be associated with the early appearance of this symptom. The study demonstrated that pregnant women with DM1 complain of pain with a moderate incidence in the second trimester of pregnancy. Factors such as length of illness are related to the higher frequency of pain in pregnant women with DM1.

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Highlights Correlation analysis of miR-141-5p and serum β-HCG, P4 and E2 levels in patients with ESA. miR-141-5p as a diagnostic biomarker for ESA. β-HCG, P4 and E2 levels.

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Highlights Tyrosine kinase domains with genomic alterations have oncogenic potential. Higher efficacy for infigratinib than larotrectinib. Infigratinib has a higher overall survival than larotrectinib. Infigratinib has higher adverse effects than larotrectinib. Bevacizumab initial therapy has a higher overall survival.

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Abstract Introduction The maternal mortality rate in developing countries, such as Brazil, has significantly increased since 2020. Obstetric Emergencies (OE) account for 72.5% of these deaths. A national survey was conducted in Brazil to evaluate how gynecologists and obstetricians deal with OE and identify the main difficulties regarding theoretical/practical knowledge and structural resources. Methods An electronic questionnaire assessing resource availability, health teams, institutional protocols, and provision of OE training courses was completed by Brazilian obstetricians. Results More than 90 % of the questionnaire respondents reported treating a pregnant and/or puerperal patient with severe morbidity and that their health network has human resources, trained professionals, and structural resources required for this type of care. However, few respondents participate in continuing education programs (36 %) or specific training for the medical team (61.41 %). The implementation rates of obstetric risk identification protocols (33.09 %), a rapid response team (46.54 %), and boxes and emergency cart assembly teams (71.68 %) were determined. Conclusion A high Maternal Mortality Ratio (MMR) may be related to disorganized healthcare systems, low implementation of risk classification protocols for the care of severe maternal and fetal conditions, and lack of access to continued/specific training programs. The Brazilian MMR is multifactorial. According to obstetricians, Brazilian health services include care teams, essential medications, obstetric centers, and clinical analysis laboratories, though they lack systematized processes and permanent professional training for qualified care of OE.

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Highlights XBJ improves LPS-induced lung cell inflammation and pyroptosis. miR-181d-5p inhibits LPS-induced inflammatory response and pyroptosis of lung epithelial cells. XBJ elevates miR-181d-5p and improves LPS-induced pyroptosis of lung epithelial cells. XBJ upregulates miR-181d-5p and inhibits SPP1 to protect lung epithelial cells from LPS-induced injuries.

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Highlights Few studies have examined outcomes after intrathoracic metastasectomy for nonseminomatous testicular germ cell tumors. Integration of local and systemic therapies yields favorable outcomes with low morbidity and mortality. Surgical management should be considered after chemotherapy, as histology prediction for metastatic lesions remains challenging. This study highlights the importance of aggressive surgical approaches in improving long-term survival in young patients with testicular NSGCT.

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Highlights Serum INSL-3 in patients with varicocele decreases with the severity of the disease. Serum INSL-3 is positively correlated with total sperm count. Serum InhB is associated with semen volume, concentration, and total sperm. Serum InhB and INSL-3 levels could distinguish the degree of varicocele.

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Highlights Patients presenting periodontal disease increased the risk of developing gastric adenocarcinoma by 17 %. The association remained regardless of the diagnostic method for periodontal disease, i.e., clinical examination and self-report. Moreover, Asian patients with periodontal disease had a higher risk of having gastric adenocarcinoma than American and European patients.

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Highlights Obstructive sleep apnea affects one billion people worldwide and is associated with cardiometabolic risk and cognitive impairment. Drug therapy for the management of sleep apnea has been investigated, but no robust evidence that supports its benefits has been found to date. The combination of noradrenergic and antimuscarinic drugs shows promising results.