Evaluation of oxidative stress in an experimental model of Crohn's disease treated with hyperbaric oxygen therapy

Nakutis, Fernanda Serafim; Nishitokukado, Iêda; dos Santos, Fabiana Maria; Ortiz-Agostinho, Carmen Lucia; de Alencar, Daniel Teixeira; Achtschin, Cassiana Ganem; Nunes, Valeria Sutti; Leite, André Zonetti Arruda; Sipahi, Aytan Miranda

doi:10.1016/j.clinsp.2023.100305

Acessibilidade / Reportar erro

Brasil

Español English

sumário « anterior atual seguinte »

Sumário

Original articles • Clinics 78 • 2023 • https://doi.org/10.1016/j.clinsp.2023.100305 copy

Evaluation of oxidative stress in an experimental model of Crohn's disease treated with hyperbaric oxygen therapy

Authorship SCIMAGO INSTITUTIONS RANKINGS

Highlights

HBO promoted a significant improvement in the clinical and histological statuses of the animals of this study experimental.
HBO is effective for the treatment of colitis by increasing the activity of antioxidant enzymes.
This study demonstrated a modulation of the immune response by reducing the expression of inflammatory cytokines and increasing the anti-inflammatory cytokines.

Abstract

Introduction: Treatments of Inflammatory Bowel Disease (IBD) are able to control symptoms in most cases, however, a fraction of patients do not improve or have a loss of response to treatments, making it important to explore new therapeutic strategies. Hyperbaric oxygen therapy (HBO) may represent one of them. The aim of this study was to evaluate the effects of HBO therapy in an experimental model of IBD.

Methods: Sixty male BALBc mice were divided into six groups. Group 1 was colitis-induced with trinitrobenzene sulfonic acid (TNBS) + ethanol, group 2 received TNBS + ethanol plus HBO, group 3 received only ethanol, group 4 received ethanol plus HBO, group 5 received saline solution, and group 6 received saline solution plus HBO. HBO was performed for four days, subsequently, the mice were evaluated daily. At the end of the study, samples from the intestine were collected for histological analysis as well as for measurement of antioxidant enzymes and cytokine levels.

Results: HBO significantly improved the clinical and histological status of the animals. Treatment with HBO increased the activity of the antioxidant enzymes superoxide dismutase (SOD) and glutathione peroxidase (GPx) in all of the groups; moreover, the difference was only significant between the TNBS and TNBS + HBO groups and treatments promoted a reduction in the proinflammatory cytokines IFN-γ, IL-12, IL-17 and TNF-α and increased the anti-inflammatory cytokines IL-4 and IL-10, with no changes in IL-13.

Conclusion: HBO effectively treats TNBS-induced colitis by increasing the activity of antioxidant enzymes and modulating cytokine profiles.

Keywords
Inflammatory bowel disease; Experimental model; Hyperbaric oxygen therapy

Group	SOD (U/g)	GPx (U/g)	GR (U/g)
TNBS	31.01 ± 2.08^a	2005.97 ± 51.57^a	1077.33 ± 73.74^a
TNBS+HBO	44.35 ± 4.00^b	3028.34 ± 78.16^b	1310.90 ± 80.04^a
ETHANOL	21.62 ± 1.59^a	1748.71 ± 134.24^a	1004.90 ± 94.67^a
ETHANOL+HBO	40.37 ± 5.00^a,b	2741.17 ± 307.72^a,b	1280.15 ± 137.66^a
SALINE	20.14 ± 0.82^a	1693.84 ± 109.38^a	998.57 ± 22.81^a
SALINE+HBO	37.64 ± 3.27^a,b	2511.85 ± 429.79^a,b	1190.97 ± 184.56^a

Faculdade de Medicina / USP Rua Dr Ovídio Pires de Campos, 225 - 6 and., 05403-010 São Paulo SP - Brazil, Tel.: (55 11) 2661-6235 - São Paulo - SP - Brazil
E-mail: clinics@hc.fm.usp.br

Acompanhe os números deste periódico no seu leitor de RSS

[1] ⁎ Corresponding author. E-mail address: aytan@usp.br (A.M. Sipahi).